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Cat. No.: HY-W058805A

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p-Aminophenyl phosphate sodium

p-Aminophenyl phosphate sodium is a substrate of alkaline phosphatase (Alkaline Phosphatase, ALP). p-Aminophenyl phosphate sodium is catalytically hydrolyzed and dephosphorylated by ALP under alkaline conditions to generate highly electroactive pAP. p-Aminophenyl phosphate sodium can be used in studies for real-time monitoring of ALP release from living cells.

For research use only. We do not sell to patients.

CAS No.: 108084-47-5
Formula: C₆H₇NNaO₄P
Molecular Weight:211.09

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

p-Aminophenyl phosphate sodium Chemical Structure

CAS No.: 108084-47-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
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Biological Activity

Description

In Vitro

p-Aminophenyl phosphate (0.2-5 mM; 10 min) sodium shows cell line-dependent K_m values in the adherent cell ALP release assay: 4.75 mM for Balb/c 3T3, 5.03 mM for A549, 10.19 mM for MCF-7, and 1.85 mM for Ht-29^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Mesenchymal stromal/stem cell osteogenic differentiation

Chemical Information

CAS No. 108084-47-5
Molecular Weight 211.09
Formula C₆H₇NNaO₄P
SMILES

O=P(OC1=CC=C(C=C1)N)(O[Na])O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Balbaied T, et al. Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells. Biosensors. 2020 Aug 11;10(8):95. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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