Parbendazole hydrochloride

Parbendazole (PBZ) hydrochloride exhibits extremely potent anti-survival activity against THP-1 acute myeloid leukemia cells, with an IC₅₀ of 0.6 nM at 48 h and an IC₅₀ of 0.3 nM at 72 h. At concentrations ≥50 nM, it effectively induces rapid monocytic differentiation of THP-1 acute myeloid leukemia cells, followed by apoptosis, which is accompanied by upregulation of the KLF4/DPYSL2A differentiation axis^[1].

Parbendazole (100 nM; 48 h) hydrochloride exerts broad and potent differentiation-inducing and pro-apoptotic effects on acute myeloid leukemia cell lines with different subtypes and genetic backgrounds, while exhibiting limited toxicity to normal human hematopoietic progenitor cells; it effectively induces monocytic differentiation, triggers apoptosis, and upregulates the KLF4/DPYSL2A axis in primary human AML-PDX cells^[1].

Parbendazole (0.01-1 μM; 48 h) hydrochloride induces the expression of the Sema3A gene and protein in normal human epidermal keratinocytes in a dose-dependent manner^[2].
Parbendazole (1 μM; 48 h) hydrochloride downregulates NGF mRNA expression and upregulates KAL‑1 mRNA expression in normal human epidermal keratinocytes (NHEKs)^[2].
Parbendazole (1 μM; 24 h) hydrochloride promotes the phosphorylation of JNK and c‑Jun in normal human epidermal keratinocytes (NHEKs)^[2].
Parbendazole (1-10 μM; 24 h) hydrochloride increases the mRNA and protein expression levels of Sema3A in an in vitro reconstructed human epidermis (RHE) model^[2].
Parbendazole hydrochloride: Wild-type Physarum polycephalum CLd-AXE tubulin completely inhibits in vitro microtubule assembly at 2-5 μM, whereas mutant BEN210-AXE tubulin retains full microtubule assembly capacity even at concentrations as high as 50 μM^[3].
Parbendazole hydrochloride inhibits the growth of wild-type Physarum polycephalum CLd‑AXE myxamoebae and the in vitro assembly of their tubulin.
Parbendazole hydrochloride exhibits significant pH-dependent ultraviolet absorption characteristics, and shows a pronounced red shift in alkaline methanol medium compared to neutral and acidic methanol conditions^[4].