Changes in aurantio-obtusin and glucoaurantio-obtusin content in Cassiae Semen via treatment with a crude enzyme extract from Aspergillus usamii

  • J Microbiol Biotechnol. 2007 Nov;17(11):1894-7.
Jong-Moon Hur  1 ,  Soon-Ho Kwon ,  Jae-Hyun So ,  Mira Jun ,  Young-Hwa Kang ,  Yu-Mi Lee ,  Kyung-Bok Lee ,  In-Koo Rhee ,  Moon-Soon Lee ,  Kyung-Sik Song
Affiliations
  • 1. School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
PMID: 18092478
Abstract

Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme Extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level (133.58 +/- 0.39 microg/mg Extract) after being incubated for 50 min at 37 degrees C, whereas the inactivated crude enzyme-treated control remained unchanged (54.13 +/- 1.33 microg/mg). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third (51.09 +/- 1.63 microg/ mg) of the untreated control (143.19 +/- 2.12 microg/mg), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.

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