A more efficient method to generate integration-free human iPS cells

  • Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591.
Keisuke Okita  1 ,  Yasuko Matsumura ,  Yoshiko Sato ,  Aki Okada ,  Asuka Morizane ,  Satoshi Okamoto ,  Hyenjong Hong ,  Masato Nakagawa ,  Koji Tanabe ,  Ken-ichi Tezuka ,  Toshiyuki Shibata ,  Takahiro Kunisada ,  Masayo Takahashi ,  Jun Takahashi ,  Hiroh Saji ,  Shinya Yamanaka
Affiliations
  • 1. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. [email protected]
PMID: 21460823 DOI: 10.1038/nmeth.1591
Abstract

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.