Inhibition of colony formation of drug-resistant human tumor cell lines by combinations of interleukin-2-activated killer cells and antitumor drugs
- Jpn J Cancer Res. 1989 Mar;80(3):265-70. doi: 10.1111/j.1349-7006.1989.tb02303.x.
- 1. Department of Internal Medicine, National Cancer Center Hospital, Tokyo.
The cytotoxicity of interleukin-2-activated killer (LAK) cells with or without Anticancer drugs against cell lines with acquired drug resistance was evaluated in vitro by colony assay. Human Non-Small Cell Lung Cancer cell lines, PC-9 and PC-14, human leukemia cell line, K-562, and their sublines resistant to cisplatin (CDDP), PC-9/CDDP and PC-14/CDDP, and to adriamycin (ADM), K-562/ADM, were used as target cells. PC-9/CDDP demonstrated a marked increase in susceptibility to killing by both peripheral blood lymphocytes (PBL) and LAK cells, as compared to the parental cell line, PC-9. The cytotoxicity of PBL and LAK cells against PC-14/CDDP and K-562/ADM was similar to that against their parental cell lines. Moreover, the combination of LAK and CDDP had a synergistic effect on PC-14 and PC-14/CDDP.