Visualizing biofilm by targeting eDNA with long wavelength probe CDr15

  • Biomater Sci. 2019 Aug 20;7(9):3594-3598. doi: 10.1039/c9bm00152b.
Haw-Young Kwon  1 ,  Jun-Young Kim ,  Xiao Liu ,  Jung Yeol Lee ,  Joey Kuok Hoong Yam ,  Louise Dahl Hultqvist ,  Wang Xu ,  Morten Rybtke ,  Tim Tolker-Nielsen ,  Wooseok Heo ,  Jong-Jin Kim ,  Nam-Young Kang ,  Taiha Joo ,  Liang Yang ,  Sung-Jin Park ,  Michael Givskov ,  Young-Tae Chang
Affiliations
  • 1. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea. [email protected].
PMID: 31329200 DOI: 10.1039/c9bm00152b
Abstract

Detection of the biofilm of bacteria would be a counter strategy to detect hidden bacteria in their camouflage. Through unbiased screening of bacteria biofilm, we discovered a long wavelength probe CDr15 with extracellular DNA as the molecular target. CDr15 revealed a real-time geometric distribution of eDNA in a 3D Bacterial colony.

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