PUN-96956

PUN-96956 (compound 2) (12-100 μM; 10 h) inhibits the growth of *Escherichia coli* MC4100 wild-type cells and cells expressing FhuA ΔC/4L in a dose-dependent manner^[1].
PUN-96956 (12-200 μM; 10 h) inhibits the growth of various Gram-negative bacteria in a dose-dependent manner^[1].

PUN-96956 (3 h) activates the σE cell envelope stress response in Escherichia coli TOP10F′ cells with an EC₅₀ of 6 μM, and does not induce intracellular heat shock stress^[1].
PUN-96956 (up to 200 μM; 10 h) exhibits enhanced growth inhibitory activity in Escherichia coli MC4100 ΔsurA, ΔbamB, and ΔdegP cells^[1].
PUN-96956 (12 μM; 3 h) reduces the steady-state levels of the β-barrel outer membrane proteins OmpF, OmpC, OmpA, and BamA in Escherichia coli TOP10F′ cells without affecting lipoprotein levels^[1].
PUN-96956 (12-25 μM; 2.5 h) reduces the de novo biogenesis of the β-barrel outer membrane protein PhoE in Escherichia coli TOP10F′ cells by interfering with membrane targeting or insertion (rather than folding) processes^[1].
PUN-96956 (12 μM; 2.5 h) impairs the secretion of the autotransporter protein Hbp in Escherichia coli MC1061 degP::S210A cells by inhibiting the assembly of the C-terminal β-barrel domain of Hbp, and simultaneously upregulates σ_E stress-regulated DegP^[1].
PUN-96956 (25-100 μM; 5 min preincubation, up to 80 min folding reaction) inhibits the BAM-dependent folding and insertion of the β-barrel protein OmpT in vitro, with an EC₅₀ of 58.3 μM. It exerts its effect primarily during the folding process rather than by directly inhibiting the protease activity of OmpT^[1].
PUN-96956 (up to 60 μM; 48 h) exhibits cytotoxicity against HEK293 human embryonic kidney cells, with an LD₅₀ of 20 μM^[1].
PUN-96956 (0.08-60 μM; 3 h) induces dose-dependent σ^E and RprA stress responses in Escherichia coli TOP10F' cells without affecting the GroES stress response; after 3 h of incubation, a maximum 2.5-fold induction of σ^E and 5-fold induction of RprA are achieved at a concentration of 60 μM^[1].
PUN-96956 (100 μM) disrupts the assembly of OmpT via the BAM complex in proteoliposomes in a dose-dependent manner, with the inhibitory effect reaching a peak at 100 μM, and does not inhibit the protease activity of fully folded OmpT^[1].