Quorum sensing-IN-11
Based on 1 Customer Validation
Quorum sensing-IN-11 is a quorum sensining inhibitor and a LasR antagonist with an IC50 of 0.7399 μM. Quorum sensing-IN-11 inhibits biofilm, pyocyanin formation and swimming motility in Pseudomonas aeruginosa. Quorum sensing-IN-11 can be used for the research of Pseudomonas aeruginosa infection.
For research use only. We do not sell to patients.
- Purity : 96.92%
- CAS No.: 3116844-25-5
- Formula: C18H12BrN3O
- Molecular Weight:366.21
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Quorum sensing-IN-11 (Compound 7f) (24 h) inhibits biofilm, pyocyanin formation and swimming motility in Pseudomonas aeruginosa by 83, 76, 90%[1].
Quorum sensing-IN-11 (0.1-10 μM; 4 h) potently inhibits LasR in the β-galactosidase reporter assay with an IC50 of 0.7399 ± 0.005 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3116844-25-5
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Appearance Solid
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Molecular Weight 366.21
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Formula C18H12BrN3O
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Color Yellow to orange
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SMILES
BrC(C=C1)=CC=C1C(/C=C/C(C#N)=C2NC3=CC=CC=C3N/2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 14.29 mg/mL (39.02 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.43 mg/mL (3.90 mM); Suspended solution
This protocol yields a suspended solution of ≥ 1.43 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7307 mL | 13.6534 mL | 27.3067 mL | 68.2668 mL |
| 5 mM | 0.5461 mL | 2.7307 mL | 5.4613 mL | 13.6534 mL | |
| 10 mM | 0.2731 mL | 1.3653 mL | 2.7307 mL | 6.8267 mL | |
| 15 mM | 0.1820 mL | 0.9102 mL | 1.8204 mL | 4.5511 mL | |
| 20 mM | 0.1365 mL | 0.6827 mL | 1.3653 mL | 3.4133 mL | |
| 25 mM | 0.1092 mL | 0.5461 mL | 1.0923 mL | 2.7307 mL | |
| 30 mM | 0.0910 mL | 0.4551 mL | 0.9102 mL | 2.2756 mL |
Quorum sensing-IN-11 Related Classifications
HY-181760 Related Classifications
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