SCP-7
SCP-7 is a STAT3 inhibitor with a Kd value of 795 nM. SCP-7 induces mitochondrial dysfunction, increases ROS levels and promotes apoptosis. SCP-7 induces G2/M phase arrest of the cell cycle. SCP-7 inhibits the proliferation, migration and colony formation of colorectal cancer cells. SCP-7 suppresses tumor growth in a mouse xenograft model of colorectal cancer. SCP-7 is applicable to research related to colorectal cancer.
For research use only. We do not sell to patients.
- CAS No.: 2479389-64-3
- Formula: C22H20O6
- Molecular Weight:380.39
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
STAT3 795 nM (Kd) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
3.84 μM
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Inhibition of cell viability against human HCT116 cells incubated for 48 hrs by MTT assay.
Inhibition of cell viability against human HCT116 cells incubated for 48 hrs by MTT assay.
|
42497765 |
| HT-29 | IC50 |
1.58 μM
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Inhibition of cell viability against human HT29 cells incubated for 48 hrs by MTT assay.
Inhibition of cell viability against human HT29 cells incubated for 48 hrs by MTT assay.
|
42497765 |
| HEK-293T | IC50 |
18.34 μM
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Inhibition of cell viability against human HEK-293T cells incubated for 48 hrs by MTT assay.
Inhibition of cell viability against human HEK-293T cells incubated for 48 hrs by MTT assay.
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42497765 |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116, HT29
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Concentration:0, 0.5, 1 and 2 μM
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Incubation Time:12 h, 24 h
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Result:Inhibited the migration.
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Cell Line:inhibits the migration
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Concentration:0, 2.5, 5 and 10 μM
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Incubation Time:48 h
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Result:Induced cell apoptosis.
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Cell Line:inhibits the migration
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Concentration:0, 0.625, 1.25, 2.5, 5 and 10 μM
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Incubation Time:48 h
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Result:Upregulated BAX.
Downregulated Bcl-2, Mcl-1, survivin and c-Myc.
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Cell Line:HCT116, HT29
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Concentration:0, 2.5, 5 and 10 μM
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Incubation Time:48 h
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Result:Increased the proportion of cells in the G2/M phase in a concentration-dependent manner in both HCT116 and HT29 cells.
Significantly reduced cyclin B1 and CDK1 expression levels in both cell lines.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nude mice[1]
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Dosage:2.5 mg/kg; 5 mg/kg
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Administration:i.p.; once every two days; 14 days
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Result:Suppressed HCT116 xenograft tumor growth in a dose-dependent manner.
Reduced tumor weight and volume significantly compared to the control group.
Reduced Ki67 and p-STAT3 levels in tumor tissue.
Increased BAX levels in tumor tissue.
Downregulated downstream STAT3 targets c-Myc and Bcl-2.
Caused no significant changes in mouse body weight during treatment.
Showed no significant toxicity via organ index analysis, H&E staining of major organs, and serum levels of ALT, AST, CRE, and TBIL.
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- BrdU Incorporation Assay
- Cell migration
- Patient-Derived Orthotopic Xenograft (PDOX)
- Protocol for Cell Cycle
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Mitochondrial membrane-potential and mitochondrial mass staining
- Patient-Derived Xenograft (PDX)
- Apoptosis Solutions
- Splenic/Portal-Vein Liver Metastasis Xenograft
- Soft Agar Colony Formation Assay
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
- Intraperitoneal/Peritoneal Dissemination Xenograft
Chemical Information
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CAS No. 2479389-64-3
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Molecular Weight 380.39
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Formula C22H20O6
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SMILES
O=C(C1=CC=CC=C1)O[C@@H]2/C=C(C)/C[C@H](OC3=O)C=C3C[C@H]4[C@H]2C(C(O4)=O)=C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SCP-7 Related Classifications
HY-187792 Related Classifications
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