2 Results for "

acyl-enzyme intermediate

" in MedChemExpress (MCE) Product Catalog:
Products (2)

2 Results for "acyl-enzyme intermediate" in MCE Product Catalog:

Cat. No.: HY-E70437
CAS No.: 80146-85-6
Synonyms: Tissue-type transglutaminase; TG2; TGase 2
Research Areas:  

Others

Guinea pig liver transglutaminase (Tissue-type transglutaminase) is a calcium-dependent tissue-type transglutaminase and serves as the prototype of intracellular tissue transglutaminases purified from guinea pig liver. Guinea pig liver transglutaminase catalyzes acyl transfer reactions via a ping-pong mechanism, forming a transient thioester acyl-enzyme intermediate that mediates the hydrolysis of glutamine amides, displacement by primary amines, and the formation of isopeptide cross-links between peptide-bound glutamine and the amino groups of lysine or polyamines .
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Cat. No.: HY-P5510
CAS No.: 188530-20-3
Synonyms: HCV NS3 protease substrate
Target:  

Inhibitory Antibodies

Research Areas:  

Others

Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 (HCV NS3 protease substrate) is a biological active peptide. (This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM.
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