Tempone-d16
Tempone-d16 (4-Oxo-Tempo-d16) is the deuterated-labeled Tempone (HY-129242). Tempone (4-Oxo-Tempo) is a nitroxide radical spin label and ROS scavenger. Tempone induces cytotoxicity in lymphoma cells. Tempone serves as a substrate for intracellular reduction to hydroxylamine, rapidly equilibrates between intracellular and extracellular compartments, reduces nitroxide radical spin labels at the ubiquinol site of the respiratory chain, and acts as an alternative terminal electron acceptor when electron flow to oxygen is blocked. Tempone serves as a superoxide sensor, a T2-weighted MRI contrast agent, and a dynamic nuclear polarization polarizing agent for 13C. Tempone inhibits superoxide and peroxynitrite, hydroxyl radical generation, nitrotyrosine formation, and poly (ADP-ribose) formation, and alleviates renal dysfunction and injury in ischemia/reperfusion and hydrogen peroxide-induced injury. Tempone can be used for research on ischemia-reperfusion injury, acute renal failure, and lymphoma.
For research use only. We do not sell to patients.
- CAS No.: 36763-53-8
- Formula: C9D16NO2-
- Molecular Weight:186.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 36763-53-8
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Unlabeled CAS 2896-70-0
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Molecular Weight 186.33
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Formula C9D16NO2-
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SMILES
[2H]C([2H])([2H])C(C([2H])([2H])[2H])(C1([2H])[2H])N([O-])C(C([2H])([2H])[2H])(C([2H])([2H])[2H])C([2H])([2H])C1=O
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Synonyms
4-Oxo-Tempo-d16
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
DMSO : 30 mg/mL (161.00 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Guo X, et al. Comparative Genotoxicity of TEMPO and 3 of Its Derivatives in Mouse Lymphoma Cells. Toxicological sciences : an official journal of the Society of Toxicology. 2018 May 01;163(1):214-225. [Content Brief]
[7]. Patel NS, et al. TEMPONE reduces renal dysfunction and injury mediated by oxidative stress of the rat kidney. Free radical biology & medicine. 2002 Dec 01;33(11):1575-89. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3668 mL | 26.8341 mL | 53.6682 mL | 134.1706 mL |
| 5 mM | 1.0734 mL | 5.3668 mL | 10.7336 mL | 26.8341 mL | |
| 10 mM | 0.5367 mL | 2.6834 mL | 5.3668 mL | 13.4171 mL | |
| 15 mM | 0.3578 mL | 1.7889 mL | 3.5779 mL | 8.9447 mL | |
| 20 mM | 0.2683 mL | 1.3417 mL | 2.6834 mL | 6.7085 mL | |
| 25 mM | 0.2147 mL | 1.0734 mL | 2.1467 mL | 5.3668 mL | |
| 30 mM | 0.1789 mL | 0.8945 mL | 1.7889 mL | 4.4724 mL | |
| 40 mM | 0.1342 mL | 0.6709 mL | 1.3417 mL | 3.3543 mL | |
| 50 mM | 0.1073 mL | 0.5367 mL | 1.0734 mL | 2.6834 mL | |
| 60 mM | 0.0894 mL | 0.4472 mL | 0.8945 mL | 2.2362 mL | |
| 80 mM | 0.0671 mL | 0.3354 mL | 0.6709 mL | 1.6771 mL | |
| 100 mM | 0.0537 mL | 0.2683 mL | 0.5367 mL | 1.3417 mL |
Tempone-d16 Related Classifications
HY-129242S1 Related Classifications
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