Thiazolyl Blue (solution)
Based on 1 Customer Validation
Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
Solvent and concentration: PBS: 5 mg/mL
For research use only. We do not sell to patients.
- CAS No.: 298-93-1
- Formula: C18H16BrN5S
- Molecular Weight:414.32
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Solvent and concentration: PBS: 5 mg/mL
In Vitro
Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
1. Cell Proliferation Assay (96-Well Plate)
1.1 Cell seeding: Prepare a single-cell suspension in culture medium containing 10% FBS and seed into a 96-well plate at 1000-10000 cells per well in a final volume of 100 μL per well.
1.2 Cell culture: Incubate cells at 37°C with 5% CO2 for 24-72 h.
1.3 Add 10 μL MTT to each well and incubate for 4 h. Discard the supernatant; for suspension cells, centrifuge before removing the supernatant.
1.4 Add 100 μL DMSO and shake for 10 minutes to completely dissolve the crystals.
1.5 Measure the increase in absorbance at OD=562 nm using a microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Cell Cytotoxicity Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 298-93-1
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Appearance Liquid
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Molecular Weight 414.32
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Formula C18H16BrN5S
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Color Light yellow to yellow
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SMILES
CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]
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Synonyms
MTT (solution); Thiazolyl Blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-tetrazolium bromide (solution)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52. [Content Brief]
[2]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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