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Cat. No.: HY-189960

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Top1-IN-3

Top1-IN-3 is a DNA topoisomerase 1 (TOP1) inhibitor. Top1-IN-3 modulates P-glycoprotein (P-gp)-mediated transport. Top1-IN-3 exhibits anticancer activity against ovarian cancer.

For research use only. We do not sell to patients.

Formula: C₃₄H₂₉N₂O₂P
Molecular Weight:528.58

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Top1-IN-3 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

Top1-IN-3 is a DNA topoisomerase 1 (TOP1) inhibitor. Top1-IN-3 modulates P-glycoprotein (P-gp)-mediated transport. Top1-IN-3 exhibits anticancer activity against ovarian cancer^[1].

IC₅₀ & Target

^[1]

Top1

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
SK-OV-3	IC50	16.49 μM	Antiproliferative activity against human SKOV-3 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay. Antiproliferative activity against human SKOV-3 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay.	42777668
A549	IC50	50 μM	Antiproliferative activity against human A549 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay; IC50 >50 μM. Antiproliferative activity against human A549 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay; IC50 >50 μM.	42777668
MRC5	IC50	50 μM	Antiproliferative activity against human MRC-5 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay; IC50 >50 μM. Antiproliferative activity against human MRC-5 cells assessed as viability reduction incubated 48 hrs by CCK-8 assay; IC50 >50 μM.	42777668
MDCK-MDR1	EC50	12.30 μM	EC50 for increased calcein-AM accumulation in MDCK-MDR1 cells overexpressing P-glycoprotein incubated 30 min with compound and 30 min with 2.5 μM calcein-AM measured by fluorescence. EC50 for increased calcein-AM accumulation in MDCK-MDR1 cells overexpressing P-glycoprotein incubated 30 min with compound and 30 min with 2.5 μM calcein-AM measured by fluorescence.	42777668

In Vitro

Anti-cancerOthers

Anti-cancer

Top1-IN-3 (48 h) selectively inhibits the growth of SKOV-3 ovarian cancer cells with an IC₅₀ of 16.49 μM, whereas the IC₅₀ in A549 and MRC-5 cells is >50 μM^[1].

Others

Top1-IN-3 (Compound 8b) (3.2 μM; 1-5 min) inhibits hTOP1-mediated DNA relaxation, with activity higher than that of CPT^[1].
Top1-IN-3 (0.1-100 μM; 30 min) modulates P-gp-mediated transport in MDCK-MDR1 cells, with an EC₅₀ of 12.30 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 528.58
Formula C₃₄H₂₉N₂O₂P
SMILES

[H][C@]12N=C3C(P(C4=CC=CC=C4)(C5=CC=CC=C5)=O)=CC=CN3[C@H]([C@@]1(COC6=C2C=C(C=C6)C)[H])C7=CC=CC=C7
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Lopez-Aguileta, et al. Exploring phosphorus substitution in chromeno[4,3-d]pyrido[1,2-a]pyrimidines: new insights into Topoisomerase 1 inhibition and cytotoxicity. Eur J Med Chem. 2026 Sep 11;320:119316.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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