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Cat. No.: HY-146307

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TrxR-IN-3

TrxR-IN-3 is a thioredoxin reductase (TrxR) inhibitor with an IC₅₀ of 0.12 µM in MDA-MB-231 cells. TrxR-IN-3 inhibits TrxR enzyme activity and suppresses TrxR protein expression. TrxR-IN-3 induces reactive oxygen species (ROS) accumulation, apoptosis, and autophagy. TrxR-IN-3 modulates apoptosis-related proteins by decreasing anti-apoptotic protein levels and increasing caspase cleavage. TrxR-IN-3 modulates autophagy-related proteins by increasing autophagy markers and reducing autophagy substrate proteins. TrxR-IN-3 can be used for breast cancer research.

For research use only. We do not sell to patients.

CAS No.: 2445565-58-0
Formula: C₁₄H₁₁F₃O₂
Molecular Weight:268.23

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (281 KB)

TrxR-IN-3 Chemical Structure

CAS No.: 2445565-58-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

TrxR

0.12 μM (IC₅₀)

All TrxR Isoforms :

TrxR1
TrxR2

Cellular Effect

Cell Line	Type	Value	Description	References
MDA-MB-231	IC50	0.59 μM	Antiproliferative activity against human MDA-MB-231 cells assessed as viability reduction incubated 72 hrs by SRB assay. Antiproliferative activity against human MDA-MB-231 cells assessed as viability reduction incubated 72 hrs by SRB assay.	32736231
MCF7	IC50	1.04 μM	Antiproliferative activity against human MCF-7 cells assessed as viability reduction incubated 72 hrs by SRB assay. Antiproliferative activity against human MCF-7 cells assessed as viability reduction incubated 72 hrs by SRB assay.	32736231
A549	IC50	0.44 μM	Antiproliferative activity against human A549 cells assessed as viability reduction incubated 72 hrs by SRB assay. Antiproliferative activity against human A549 cells assessed as viability reduction incubated 72 hrs by SRB assay.	32736231
KB	IC50	0.53 μM	Antiproliferative activity against human KB cells assessed as viability reduction incubated 72 hrs by SRB assay. Antiproliferative activity against human KB cells assessed as viability reduction incubated 72 hrs by SRB assay.	32736231
KB-V1	IC50	0.84 μM	Antiproliferative activity against human KB-VIN cells assessed as viability reduction incubated 72 hrs by SRB assay. Antiproliferative activity against human KB-VIN cells assessed as viability reduction incubated 72 hrs by SRB assay.	32736231

In Vitro

Anti-cancerApoptosisOxidative StressAutophagyOthers

Anti-cancer

TrxR-IN-3 (compound 2c) (72 h) inhibits MDA-MB-231 breast cancer cell proliferation with an IC₅₀ of 0.59 µM^[1].
TrxR-IN-3 (0.01-5.0 µM; 72 h) inhibits TrxR activity in MDA-MB-231 cells with an IC₅₀ of 0.12 µM^[1].
TrxR-IN-3 (0.2-1.8 µM; 72 h) inhibits TrxR1 protein expression in MDA-MB-231 cells after 72 h^[1].

Anti-cancer

Apoptosis

TrxR-IN-3 (0.2-1.8 µM; 72 h) regulates apoptosis-related proteins in MDA-MB-231 cells^[1].
TrxR-IN-3 (0.2-1.8 µM; 72 h) induced apoptosis in MDA-MB-231 cells, with apoptotic cells reaching 56.8% at 1.8 µM^[1].

Oxidative Stress

Anti-cancer

TrxR-IN-3 (0.01-10 μM; 24 h) increases ROS levels in MDA-MB-231 cells^[1].

Anti-cancer

Autophagy

TrxR-IN-3 (0.6-1.8 µM; 24 h) dose-dependently induces the accumulation of autophagosomes and autolysosomes in MDA-MB-231 cells^[1].
TrxR-IN-3 (72 h) regulates autophagy-related proteins in MDA-MB-231 cells^[1].

Others

TrxR-IN-3 inhibits purified TrxR enzyme in the DTNB assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0.2, 0.6, 1.8 µM
Incubation Time:

72 h
Result:

Diminished TrxR1 protein expression dose-dependently.\n
Reduced Bcl-2 and Bcl-xL expression dose-dependently and caused cleavage of caspase-3.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0.2, 0.6, 1.8 µM
Incubation Time:

72 h
Result:

Induced apoptosis with apoptotic rates of 20.7%, 45.6%, and 56.8% at 0.2, 0.6, and 1.8 µM, respectively.

Cell Autophagy Assay^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0.6, 1.8 µM
Incubation Time:

24 h
Result:

Increased red and yellow fluorescence spots dose-dependently, representing autophagosomes and autolysosomes.

In Vivo

Anti-cancer

TrxR-IN-3 (compound 2c) (10-25 mg/kg; i.p.; 21 days) significantly inhibits breast tumor growth in MDA-MB-231 xenograft mice, reducing tumor mass by 63.3% at 25 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude (female, 5-6 weeks old)^[1]
Dosage:

10 mg/kg; 25 mg/kg
Administration:

i.p.; 21 days
Result:

Reduced tumor mass by 63.3% (0.57 g vs 1.56 g) at 25 mg/kg.

Related Protocols :

Chemical Information

CAS No. 2445565-58-0
Molecular Weight 268.23
Formula C₁₄H₁₁F₃O₂
SMILES

O=C1C=CCC/C1=C\C2=CC=C(OC(F)(F)F)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Qian J, et al. Design and synthesis of benzylidenecyclohexenones as TrxR inhibitors displaying high anticancer activity and inducing ROS, apoptosis, and autophagy. European journal of medicinal chemistry. 2020 Oct 15;204:112610. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References

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TrxR-IN-3

All TrxR Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

In Vivo

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

TrxR-IN-3 Related Classifications

HY-146307 Related Classifications

Bioz

Keywords

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