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Cat. No.: HY-189307

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WEE1-IN-15

WEE1-IN-15 is an ATP-competitive, selective WEE1 kinase inhibitor (IC₅₀: 42 nM). WEE1-IN-15 abolishes CDK1 phosphorylation. WEE1-IN-15 induces DNA damage and replication stress, and increases the S-phase cell population. WEE1-IN-15 shows selective anticancer effects on colorectal cancer organoids.

For research use only. We do not sell to patients.

Formula: C₂₉H₃₄N₈O₃
Molecular Weight:542.63

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

WEE1-IN-15 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

CDK1

All Wee1 Isoforms :

PKMYT1

All CDK Isoforms :

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	EC50	15 nM	Cellular target engagement of WEE1 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay. Cellular target engagement of WEE1 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay.	42623604
HEK-293T	EC50	660 nM	Cellular target engagement of WEE2 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay. Cellular target engagement of WEE2 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay.	42623604
HEK-293T	EC50	500 nM	Cellular target engagement of MAP3K4 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay. Cellular target engagement of MAP3K4 in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay.	42623604
HEK-293T	EC50	415 nM	Cellular target engagement of TNNI3K in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay. Cellular target engagement of TNNI3K in HEK293T cells transfected with NanoLuc fusion constructs incubated for 2 hrs by NanoBRET assay.	42623604

In Vitro

Anti-cancerOthers

Anti-cancer

WEE1-IN-15 (100 pM-100 μM; 5 days) exhibits potent anticancer activity against colorectal cancer organoids (IC₅₀ of 164 nM for PM003, 169 nM for QEH039LM, and 171 nM for RAH051T)^[1].

Others

WEE1-IN-15 (Compound 2) potently inhibits WEE1 with an IC₅₀ of 42 nM and displays 38-fold selectivity over PLK1^[1].
WEE1-IN-15 effectively binds WEE1 in cells with an EC₅₀ of 15 nM and shows ≥28-fold selectivity over the off-target kinases WEE2, MAP3K4, and TNNI3K^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

Molecular Weight 542.63
Formula C₂₉H₃₄N₈O₃
SMILES

CC(C)(O)C1=NC(N2C(N=C(NC3=CC4=C(N5CCN(C)CC5)C=C3)N=C6)=C6C(N2CC=C)=O)=CC(COC4)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Syphers JL, et al. Selective Macrocyclic WEE1 Kinase Inhibitors with Strong Efficacy against Patient-Derived Colorectal Cancer Organoids. Journal of medicinal chemistry. 2026 Sep 10;69(17):20160-20187.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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WEE1-IN-15

All Wee1 Isoforms

All CDK Isoforms

All DNA/RNA Synthesis Isoforms