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Cat. No.: HY-115736

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Xanthosine-5'-triphosphate

Name: Xanthosine-5'-triphosphate
Brand: MedChemExpress
SKU: HY-115736
Availability: PreOrder

(Synonyms: 5'-XTP)

Xanthosine-5'-triphosphate (5'-XTP) is a purine nucleotide that stimulates Adenylate Cyclase to induce Cyclic AMP (cAMP) (HY-B1511) biosynthesis, binds to the AL1 allosteric site of the D137N-SAMHD1 mutant to activate dNTP triphosphohydrolase activity, and stimulates P2Y/P2U purinergic receptor-coupled phosphoinositide-specific phospholipase C with Michaelis-Menten kinetics. Xanthosine-5'-triphosphate can be used for HIV-1 research.

For research use only. We do not sell to patients.

CAS No.: 6253-56-1
Formula: C₁₀H₁₅N₄O₁₅P₃
Molecular Weight:524.17

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (286 KB)

Xanthosine-5'-triphosphate Chemical Structure

CAS No.: 6253-56-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC50	139 μM	Inhibition of (-)-isoproterenol-stimulated GTP hydrolysis in Sf9 cell membranes expressing the β2-adrenergic receptor fused to the long splice variant of Gsα (β2ARGsαL) incubated for 20 min at 25°C by charcoal slurry-based GTPase assay. Inhibition of (-)-isoproterenol-stimulated GTP hydrolysis in Sf9 cell membranes expressing the β2-adrenergic receptor fused to the long splice variant of Gsα (β2ARGsαL) incubated for 20 min at 25°C by charcoal slurry-based GTPase assay.	39632506

In Vitro

Anti-cancerOthers

Anti-cancer

Xanthosine-5'-triphosphate (5'-XTP) (10 min) is a potent agonist of phospholipase C in 1321N1 human astrocytoma cells expressing the avian tp2y receptor, with an EC₅₀ of 253 nM^[7].
Xanthosine-5'-triphosphate (10 min) is a potent agonist that inhibits adenylyl cyclase in 1321N1 human astrocytoma cells expressing the avian tp2y receptor, with an EC₅₀ of 172 nM^[7].

Others

Xanthosine-5'-triphosphate (Various concentrations; 30 min) stimulates phosphoinositide phospholipase C in the ampullary epithelium of the posterior vertical semicircular canal of Rana ridibunda with a pK_a of 3.59, an nH of 1.03, and an intrinsic activity of 0.9 relative to 1 mM ATP^[3].
Xanthosine-5'-triphosphate is identified as a substrate of inosine triphosphate pyrophosphatase protein in the 2DE-CETSA dataset of rat hippocampal cytosolic fraction^[5].
Xanthosine-5'-triphosphate (100 µM; 20 min) minimally increases basal adenylyl cyclase activity in Sf9 membranes expressing β₂ARGsαL, but supports agonist-stimulated adenylyl cyclase activity with lower potency than GTP and ITP^[6].
Xanthosine-5'-triphosphate's hydrolysis (20 min) in Sf9 membranes is caused by endogenous nucleotidases, Gsα does not hydrolyze XTP to a measurable extent, and XTP hydrolysis is not regulated by β₂AR ligands^[6].
Xanthosine-5'-triphosphate (139 µM; 20 min) blocks (-)-ISO-stimulated GTP hydrolysis in Sf9 membranes expressing β₂ARGsαL, indicating that XTP binds to Gsα but is not hydrolyzed^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Xanthosine-5'-triphosphate (0.2 M; iontophoresis) is largely devoid of depressant activity on cerebral cortical neurons (12/50 depressed) and produces excitant actions on quiescent neurons (11/33 excited), consistent with the excitant activity shared by all purine and pyrimidine 5'-triphosphates tested^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (adult male, 250-350 g, halothane/methoxyflurane-nitrous oxide anaesthesia)^[4]
Dosage:

0.2 M
Administration:

iontophoresis
Result:

Depressed 12 of 50 neurons tested.
Excited 11 of 33 quiescent or slowly firing neurons tested.

Chemical Information

CAS No. 6253-56-1
Molecular Weight 524.17
Formula C₁₀H₁₅N₄O₁₅P₃
SMILES

O=C(C(N=C1)=C(N2)N1[C@@H]3O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)NC2=O
Synonyms

5'-XTP
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wincek TJ, et al. Effects of prostaglandins and catecholamines on rat spleen adenylate cyclase in vitro. Biochimica et biophysica acta. 1976 Jul 21;437(2):571-6.

[2]. Morris ER, et al. Crystal structures of SAMHD1 inhibitor complexes reveal the mechanism of water-mediated dNTP hydrolysis. Nature communications. 2020 Jun 23;11(1):3165.

[3]. Butlen D, et al. Purine and pyrimidine nucleotide-sensitive phosphoinositidase C in ampulla from frog semicircular canal. The American journal of physiology. 1997 Jan;272(1 Pt 2):R51-8.

[4]. Phillis JW, et al. Effects of adenosine and adenine nucleotides on synaptic transmission in the cerebral cortex. Canadian journal of physiology and pharmacology. 1979 Nov;57(11):1289-312.

[5]. Tsverava L, et al. Preventing epileptogenesis by interaction between inositol isomers and proteins. Epilepsia open. 2025 Feb;10(1):120-133.

[6]. Seifert R, et al. Effects of guanine, inosine, and xanthine nucleotides on beta(2)-adrenergic receptor/G(s) interactions: evidence for multiple receptor conformations. Molecular pharmacology. 1999 Aug;56(2):348-58.

[7]. Boyer JL, et al. A molecularly identified P2Y receptor simultaneously activates phospholipase C and inhibits adenylyl cyclase and is nonselectively activated by all nucleoside triphosphates. Molecular pharmacology. 2000 Apr;57(4):805-10.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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