1,1'-(Azodicarbonyl)-dipiperidine
Based on 1 Customer Validation
1,1'-(Azodicarbonyl)-dipiperidine (ADDP; SR 4077) is an oxidizing reagent for intracellular reducing agents and a chemical coupling reagent. 1,1'-(Azodicarbonyl)-dipiperidine exhibits biological activities such as pro-oxidation, radiosensitization, and induction of apoptosis, and can serve as a Mitsunobu reaction catalyst for chemical synthesis. 1,1'-(Azodicarbonyl)-dipiperidine oxidizes and depletes intracellular reducing pools such as GSH, leading to protein thiol oxidation and oxidative stress, thereby inhibiting DNA single-strand break repair or altering membrane function, ultimately mediating apoptosis signaling and increasing radiosensitivity. 1,1'-(Azodicarbonyl)-dipiperidine can be used in research on pulmonary arterial hypertension, lymphoma, and teratocarcinoma.
For research use only. We do not sell to patients.
- Purity : 97.0%
- CAS No.: 10465-81-3
- Formula: C12H20N4O2
- Molecular Weight:252.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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10(R)-PAHSAEndogenous Lipid
Biological Activity
Description
IC50 & Target
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PPARα |
PPAR-γ |
PPARδ |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CHO-TO
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Concentration:300 µM
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Incubation Time:Incubated for 15 or 30 minutes after X-ray irradiation, then washed and replated
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Result:Exhibited significant radiosensitizing activity and increases the cell killing rate when added after X-ray irradiation.
Chemical Information
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CAS No. 10465-81-3
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Appearance Solid
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Molecular Weight 252.31
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Formula C12H20N4O2
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Color Light yellow to yellow
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SMILES
O=C(/N=N/C(N1CCCCC1)=O)N2CCCCC2
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Synonyms
ADDP; SR 4077
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 33.33 mg/mL (132.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.9634 mL | 19.8169 mL | 39.6338 mL | 99.0845 mL |
| 5 mM | 0.7927 mL | 3.9634 mL | 7.9268 mL | 19.8169 mL | |
| 10 mM | 0.3963 mL | 1.9817 mL | 3.9634 mL | 9.9084 mL | |
| 15 mM | 0.2642 mL | 1.3211 mL | 2.6423 mL | 6.6056 mL | |
| 20 mM | 0.1982 mL | 0.9908 mL | 1.9817 mL | 4.9542 mL | |
| 25 mM | 0.1585 mL | 0.7927 mL | 1.5854 mL | 3.9634 mL | |
| 30 mM | 0.1321 mL | 0.6606 mL | 1.3211 mL | 3.3028 mL | |
| 40 mM | 0.0991 mL | 0.4954 mL | 0.9908 mL | 2.4771 mL | |
| 50 mM | 0.0793 mL | 0.3963 mL | 0.7927 mL | 1.9817 mL | |
| 60 mM | 0.0661 mL | 0.3303 mL | 0.6606 mL | 1.6514 mL | |
| 80 mM | 0.0495 mL | 0.2477 mL | 0.4954 mL | 1.2386 mL | |
| 100 mM | 0.0396 mL | 0.1982 mL | 0.3963 mL | 0.9908 mL |
1,1'-(Azodicarbonyl)-dipiperidine Related Classifications
HY-Y1624 Related Classifications
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Keywords
- 1,1'-(Azodicarbonyl)-dipiperidine
- 10465-81-3
- ADDP
- SR 4077
- SR4077
- SR 4077
- SR-4077
- Biochemical Assay Reagents
- Apoptosis
- NOS cofactor
- internucleosomal DNA fragmentation
- lymphoma
- nitric oxide formation
- protein thiol oxidation
- pulmonary artery rings
- pulmonary hypertension
- teratocarcinoma
- tetrahydrobiopterin
- vasorelaxation
- Inhibitor
- inhibitor
- inhibit