3'-Deoxyinosine
Based on 1 Customer Validation
3'-Deoxyinosine is an antiprotozoal purine nucleoside and a metabolite of Cordycepin (HY-N0262). 3'-Deoxyinosine inhibits inflammatory gene expression. 3'-Deoxyinosine inhibits the growth of Leishmania tropica promastigotes. 3'-Deoxyinosine can be used in research on cutaneous leishmaniasis.
For research use only. We do not sell to patients.
- Purity : 99.88%
- CAS No.: 13146-72-0
- Formula: C10H12N4O4
- Molecular Weight:252.23
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[5]|
Leishmania |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U-937 | EC50 |
150 μM
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Cytotoxicity against human U-937 macrophages assessed after 7 days incubation by semi-logarithmic plot of percent growth inhibition versus concentration.
Cytotoxicity against human U-937 macrophages assessed after 7 days incubation by semi-logarithmic plot of percent growth inhibition versus concentration.
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1859443 |
| FM3A | EC50 |
125 μM
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Growth inhibition against mouse mammary tumor FM3A cells assessed as concentration inhibiting growth rate by 50% of control after 48 hrs incubation.
Growth inhibition against mouse mammary tumor FM3A cells assessed as concentration inhibiting growth rate by 50% of control after 48 hrs incubation.
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6487305 |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:mouse mammary tumor FM3A cells
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Concentration:1.25 × 10^-4 M (EC50)
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Incubation Time:48 hr
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Result:EC50 for 3'-deoxyinosine against FM3A cells was 125 μM.
The ratio of EC50 values of 3'-deoxyinosine towards L. tropica and FM3A cells was 0.0035.
Chemical Information
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CAS No. 13146-72-0
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Appearance Solid
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Molecular Weight 252.23
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Formula C10H12N4O4
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Color White to off-white
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SMILES
O=C1C2=C(N([C@H]3[C@H](O)C[C@@H](CO)O3)C=N2)N=CN1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : ≥ 50 mg/mL (198.23 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (4.96 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (4.96 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9646 mL | 19.8232 mL | 39.6464 mL | 99.1159 mL |
| 5 mM | 0.7929 mL | 3.9646 mL | 7.9293 mL | 19.8232 mL | |
| 10 mM | 0.3965 mL | 1.9823 mL | 3.9646 mL | 9.9116 mL | |
| 15 mM | 0.2643 mL | 1.3215 mL | 2.6431 mL | 6.6077 mL | |
| 20 mM | 0.1982 mL | 0.9912 mL | 1.9823 mL | 4.9558 mL | |
| 25 mM | 0.1586 mL | 0.7929 mL | 1.5859 mL | 3.9646 mL | |
| 30 mM | 0.1322 mL | 0.6608 mL | 1.3215 mL | 3.3039 mL | |
| 40 mM | 0.0991 mL | 0.4956 mL | 0.9912 mL | 2.4779 mL | |
| 50 mM | 0.0793 mL | 0.3965 mL | 0.7929 mL | 1.9823 mL | |
| 60 mM | 0.0661 mL | 0.3304 mL | 0.6608 mL | 1.6519 mL | |
| 80 mM | 0.0496 mL | 0.2478 mL | 0.4956 mL | 1.2389 mL | |
| 100 mM | 0.0396 mL | 0.1982 mL | 0.3965 mL | 0.9912 mL |
3'-Deoxyinosine Related Classifications
HY-151414 Related Classifications
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