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Cat. No.: HY-134386

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6-PhEt-ATP-γ-S

Name: 6-PhEt-ATP-γ-S
Brand: MedChemExpress
SKU: HY-134386
Availability: PreOrder

(Synonyms: N6-(Phenethyl)-ATPγS)

6-PhEt-ATP-γ-S (N6-(Phenethyl)-ATPγS) is a bulky ATP analog probe carrying a thiophosphate group. 6-PhEt-ATP-γ-S, as an orthogonal phosphate donor, is specifically utilized by mutant-sensitive kinases such as CDK12-AS and Slt2-as to label their direct substrate proteins. 6-PhEt-ATP-γ-S specifically binds to the enlarged pocket of mutant kinases via its bulky side chain (avoiding wild-type), and covalently labels substrates through thiophosphorylation. 6-PhEt-ATP-γ-S can be used for kinase-substrate profiling studies.

For research use only. We do not sell to patients.

CAS No.: 944834-43-9
Formula: C₁₈H₂₄N₅O₁₂P₃S
Molecular Weight:627.40

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

6-PhEt-ATP-γ-S Chemical Structure

CAS No.: 944834-43-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
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Biological Activity

Description

In Vitro

6-PhEt-ATP-γ-S (N6-(Phenethyl)-ATPγS) (100 μM; 2 h) enables in situ thiophosphorylation and identifies 110 CDK12-dependent phosphorylation sites across 65 proteins in isolated HCT116 CDK12-AS nuclei, including Pol II CTD S1913/S1927^[1].
6-PhEt-ATP-γ-S (1 mM; 30 min) supports CDK12-AS/CCNK-mediated thiophosphorylation of recombinant LEO1 in a cell-free assay^[1].
6-PhEt-ATP-γ-S (10 mM; 30 min) is specifically utilized by Slt2-as kinase in a cell-free system (an in vitro kinase system containing yeast extract or E. coli recombinant protein) to transfer the thiophosphate group to substrate proteins (such as Rlm1, Msg5, Rcn2, Gga1, Caf20, etc.)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 944834-43-9
Molecular Weight 627.40
Formula C₁₈H₂₄N₅O₁₂P₃S
SMILES

O[C@@H]1[C@H](O)[C@@H](COP(O)(OP(O)(OP(S)(O)=O)=O)=O)O[C@H]1N2C=NC3=C(NCCC4=CC=CC=C4)N=CN=C32
Synonyms

N6-(Phenethyl)-ATPγS
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Qiu M, et al. CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation. Science advances. 2023 May 19;9(20):eadf8698.

[2]. Alonso-Rodríguez E, et al. An Analog-sensitive Version of the Protein Kinase Slt2 Allows Identification of Novel Targets of the Yeast Cell Wall Integrity Pathway. The Journal of biological chemistry. 2016 Mar 11;291(11):5461-5472.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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