7-Hydroxyisoflavone
Based on 1 Customer Validation
7-Hydroxyisoflavone is an enterovirus replication inhibitor. 7-Hydroxyisoflavone inhibits enterovirus 71 viral RNA synthesis, viral protein synthesis, and early replication steps. 7-Hydroxyisoflavone also inhibits coxsackievirus replication. 7-Hydroxyisoflavone enhances AMPK activity and prevents serum-induced reduction of AMPK phosphorylation. 7-Hydroxyisoflavone can be used in research related to enterovirus infection.
For research use only. We do not sell to patients.
- Purity : 99.75%
- CAS No.: 13057-72-2
- Formula: C15H10O3
- Molecular Weight:238.24
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | CC50 |
87.03 μM
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Cytotoxicity against African green monkey Vero cells assessed as 50% inhibition of cellular growth after 3 days incubation by Reed and Muench method.
Cytotoxicity against African green monkey Vero cells assessed as 50% inhibition of cellular growth after 3 days incubation by Reed and Muench method.
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23464760 |
In Vitro
7-Hydroxyisoflavone (1.05-8.39 μM; 24 h) inhibits EV71 (SHZH98) viral RNA synthesis in Vero cells in a dose-dependent manner[1].
7-Hydroxyisoflavone (1.05-16.79 μM; 24 h) reduces EV71 (SHZH98) VP1 protein synthesis in Vero cells in a dose-dependent manner[1].
7-Hydroxyisoflavone (3.11 μM; 0-6 h) acts on the early steps of EV71 (SHZH98) replication in Vero cells and exhibits significant antiviral activity[1].
7-Hydroxyisoflavone (48 h) inhibits coxsackieviruses B2, B3, and B6 in Vero cells, with IC50 values of 13.94, 17.04, and 10.03 μM[1].
7-Hydroxyisoflavone (40 μM) activates AMPK in a cell-free AMPK α1β1γ1 kinase assay, producing 108% relative AMPK activity[2].
7-Hydroxyisoflavone (40 μM; 30 min) activates AMPK in 3T3L1 preadipocytes by preventing the reduction of 10% fetal bovine serum-induced AMPK Thr172 phosphorylation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Vero cells infected with EV71 (SHZH98)
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Concentration:1.05, 2.1, 4.2 and 8.39 μM
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Incubation Time:24 h
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Result:Inhibited EV71 viral RNA synthesis in a dose-dependent manner.
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Cell Line:Vero cells
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Concentration:1.05, 4.2 and 16.79 μM
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Incubation Time:24 h
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Result:Reduced EV71 (SHZH98) VP1 protein synthesis in a dose-dependent manner.
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Cell Line:mouse 3T3L1 preadipocytes
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Concentration:40 μM
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Incubation Time:30 min
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Result:Serum starvation for 24 h increased AMPK phosphorylation at Thr172.
Incubation with 10% fetal bovine serum substantially reduced this serum-starvation-induced increase.
When serum-starved cells received 10% fetal bovine serum in the presence of this compound (40 μM), the serum-induced decrease in AMPK phosphorylation was prevented.
Chemical Information
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CAS No. 13057-72-2
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Appearance Solid
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Molecular Weight 238.24
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Formula C15H10O3
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Color White to off-white
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SMILES
O=C1C(C2=CC=CC=C2)=COC3=C1C=CC(O)=C3
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (209.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1974 mL | 20.9872 mL | 41.9745 mL | 104.9362 mL |
| 5 mM | 0.8395 mL | 4.1974 mL | 8.3949 mL | 20.9872 mL | |
| 10 mM | 0.4197 mL | 2.0987 mL | 4.1974 mL | 10.4936 mL | |
| 15 mM | 0.2798 mL | 1.3991 mL | 2.7983 mL | 6.9957 mL | |
| 20 mM | 0.2099 mL | 1.0494 mL | 2.0987 mL | 5.2468 mL | |
| 25 mM | 0.1679 mL | 0.8395 mL | 1.6790 mL | 4.1974 mL | |
| 30 mM | 0.1399 mL | 0.6996 mL | 1.3991 mL | 3.4979 mL | |
| 40 mM | 0.1049 mL | 0.5247 mL | 1.0494 mL | 2.6234 mL | |
| 50 mM | 0.0839 mL | 0.4197 mL | 0.8395 mL | 2.0987 mL | |
| 60 mM | 0.0700 mL | 0.3498 mL | 0.6996 mL | 1.7489 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5247 mL | 1.3117 mL | |
| 100 mM | 0.0420 mL | 0.2099 mL | 0.4197 mL | 1.0494 mL |
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