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Cat. No.: HY-134271

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8-Br-cGMP-AM

8-Br-cGMP-AM is a cGMP analog. 8-Br-cGMP-AM is a membrane-permeable, bioactivatable acetoxymethyl ester designed to avoid rapid cGMP turnover. 8-Br-cGMP-AM mediates the phosphorylation of CRP4. 8-Br-cGMP-AM has anticancer activity against leukemia. 8-Br-cGMP-AM can be used for research on acute myeloid leukemia.

For research use only. We do not sell to patients.

CAS No.: 272445-71-3
Formula: C₁₃H₁₅BrN₅O₉P
Molecular Weight:496.16

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (287 KB)

8-Br-cGMP-AM Chemical Structure

CAS No.: 272445-71-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
DRG neuron	EC50	3.7 μM	Half-maximal induction (EC50) of CRP4 phosphorylation in cultured rat DRG neurons after 30 min incubation with 8-Br-cGMP-AM measured by quantitative high-content imaging. Half-maximal induction (EC50) of CRP4 phosphorylation in cultured rat DRG neurons after 30 min incubation with 8-Br-cGMP-AM measured by quantitative high-content imaging.	40460194

In Vitro

Anti-cancerOthers

Anti-cancer

8-Br-cGMP-AM (24 h) induces cell death in rat IPC-81 leukemia cells with an EC₅₀ of 80 µM^[3].

Others

8-Br-cGMP-AM (10 μM; 120 min) induced CRP4 phosphorylation in cultured DRG neurons from WT mice, but did not induce CRP4 phosphorylation in cultured DRG neurons from Crip2^−/− mice, and induced VASP phosphorylation in both genotypes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Rat IPC-81 leukemia cells
Concentration:

Various concentrations
Incubation Time:

24 h
Result:

Induced cell death with an EC₅₀ of 80 µM after 24 h.

Related Protocols :

Chemical Information

CAS No. 272445-71-3
Molecular Weight 496.16
Formula C₁₃H₁₅BrN₅O₉P
SMILES

O[C@@H]1[C@H](OP2(OCOC(C)=O)=O)[C@@H](CO2)O[C@H]1N3C(Br)=NC4=C3N=C(N)NC4=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gerninghaus H, et al. Nociceptor-specific signaling of the receptor guanylyl cyclase Npr2 contributes to acute and persistent pain. Science signaling. 2025 Jun 03;18(889):eadq4238.

[2]. Butt E, et al. cGMP-dependent protein kinase modulators. Handbook of experimental pharmacology. 2009. [Content Brief]

[3]. Schwede F, et al. Membrane-permeant, bioactivatable analogues of cGMP as inducers of cell death in IPC-81 leukemia cells. Bioorganic & medicinal chemistry letters. 2000 Mar 20;10(6):571-3.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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