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Cat. No.: HY-P12221

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Abz-LVEALYQ-EDDnp

Abz-LVEALYQ-EDDnp is a fluorescence resonance energy transfer (FRET) oligopeptide substrate containing a peptide bond that is specifically recognized and cleaved by snake venom metalloproteinases (SVMP). Abz-LVEALYQ-EDDnp is specifically hydrolyzed by SVMP, resulting in the physical separation of the Abz fluorophore and the EDDnp quencher, which relieves the quenching effect and releases a detectable fluorescent signal.. Abz-LVEALYQ-EDDnp is used for research on inflammatory pain and antivenom (Ex/Em = 320-340/420-440 nm).

For research use only. We do not sell to patients.

CAS No.: 1000184-52-0
Formula: C₅₄H₇₅N₁₃O₁₆
Molecular Weight:1162.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Custom Peptide Synthesis

Abz-LVEALYQ-EDDnp Chemical Structure

CAS No.: 1000184-52-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

In Vitro

Abz-LVEALYQ-EDDnp (30-250 μM; 1 min) is specifically hydrolyzed in a cell-free system and is used to evaluate the inhibitory activity of broad-spectrum metalloproteinase inhibitors^[1].
Abz-LVEALYQ-EDDnp (47 mM; 30 min) is a low-molecular-weight FRET substrate that directly interacts with the active-site cleft of Atroxlysin-I and is not completely protected from hydrolysis by antibodies targeting regions distant from the catalytic site^[2].
Abz-LVEALYQ-EDDnp is incubated with Atr-I in a cell-free system to detect the neutralizing protective effect of anti-CPEN and anti-CNEN mouse serum^[3].
Abz-LVEALYQ-EDDnp (30 min) is hydrolyzed by five different Bothrops snake venoms in a cell-free system to quantify the metalloproteinase (SVMP) activity of these venoms^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Formalin-Induced Paw Inflammation/Nociceptive Inflammation

Chemical Information

CAS No. 1000184-52-0
Molecular Weight 1162.25
Formula C₅₄H₇₅N₁₃O₁₆
Sequence

{Abz}-Leu-Val-Glu-Ala-Leu-Tyr-Gln-{EDDnp}
Sequence Shortening

{Abz}-LVEALYQ-{EDDnp}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. de Souza RA, et al. Assessing the Interactions between Snake Venom Metalloproteinases and Hydroxamate Inhibitors Using Kinetic and ITC Assays, Molecular Dynamics Simulations and MM/PBSA-Based Scoring Functions. ACS omega. 2024 Dec 24;9(51):50599-50621.

[2]. Schneider FS, et al. Identification of protective B-cell epitopes of Atroxlysin-I: A metalloproteinase from Bothrops atrox snake venom. Vaccine. 2016 Mar 29;34(14):1680-7.

[3]. Kozlova EEG, et al. Computational B-cell epitope identification and production of neutralizing murine antibodies against Atroxlysin-I. Scientific reports. 2018 Oct 08;8(1):14904.

[4]. Dale CS, et al. The C-terminus of murine S100A9 inhibits hyperalgesia and edema induced by jararhagin. Peptides. 2004 Jan;25(1):81-9.

[5]. Guerra-Duarte C, et al. Partial in vitro analysis of toxic and antigenic activities of eleven Peruvian pitviper snake venoms. Toxicon : official journal of the International Society on Toxinology. 2015 Dec 15;108:84-96.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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