Acetyl-pepstatin
Acetyl-pepstatin is an aspartic protease (PRs) inhibitor that targets HIV-1 protease and XMRV protease with Ki values of 13 nM and 712 nM, respectively. Acetyl-pepstatin inhibits XMRV protease by blocking cleavage through mixed-mode binding. Acetyl-pepstatin can be used in research related to HIV-1 and XMRV infection.
For research use only. We do not sell to patients.
- CAS No.: 11076-29-2
- Formula: C31H57N5O9
- Molecular Weight:643.81
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
HIV-1 13 nM (Ki, HIV-1 protease) |
XMRV protease 712 nM (Ki) |
In Vitro
Acetyl-pepstatin (3.0 μM; 1 h) produces 50% inhibition of XMRV PR-mediated cleavage of recombinant MLV Gag fragments in SDS-PAGE experiments[1].
Acetyl-pepstatin binds XMRV PR in a mixed mode, involving two half-occupied head-to-head molecules and one half-occupied standard-mode molecule, as determined by the 2.0 Å crystal structure PDB 4EXH[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 11076-29-2
-
Molecular Weight 643.81
-
Formula C31H57N5O9
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Acetyl-pepstatin Related Classifications
HY-P1436 Related Classifications
Powered by Bioz