AIM4

AIM4 (1:10-1:15 protein:compound) inhibits in vitro TDP-43^2C amyloid aggregation in the ThT assay, with an inhibition rate of up to 76% at a 1:10 protein:compound ratio and complete inhibition at a 1:15 ratio^[1].

AIM4 (1-2 mM; preincubation for 1 h followed by LPS exposure for 24 h) reduces total nitrite production in LPS-stimulated BV2 microglial cells, indicating an anti-inflammatory effect^[2].

AIM4 (1-2 mM; pre-exposure for 1 h followed by LPS exposure for 24 h) does not significantly restore mitochondrial membrane potential in LPS-stimulated BV2 microglia^[2].

AIM4 (1:10 protein:compound) prevents TDP-43^2C from maturing into fibrils, leaving only oligomeric structures of approximately 5-10 nm at a 1:10 protein:compound ratio^[1].
AIM4 (50-200 μM; 4 h) reduces the formation of TDP-43-YFP punctate aggregates in S. cerevisiae erg6Δ cells, decreasing from approximately 20% to approximately 10% after 4 h under 0.01% galactose conditions, and from approximately 50% to approximately 25% under 0.1% galactose conditions^[1].
AIM4 (200 μM; 2 h) dissolves preformed TDP-43-YFP punctate aggregates in S. cerevisiae erg6Δ cells, reducing the proportion of cells containing aggregates from 35.5% to 22.56%^[1].
AIM4 (200 μM; 4 h) does not alter TDP-43-YFP expression levels or yeast viability under these conditions, although yeast expressing TDP-43-YFP grows relatively faster in the presence of AIM4^[1].
AIM4 (1-2 mM; 1 h) is internalized by BV2 microglial cells, but its uptake is lower than that of ABA and ABE^[2].
AIM4 (50 mM stock solution in 25% DMSO; protein:AIM4 1:15; 37°C overnight) completely inhibits the amyloid aggregation of recombinant TDP-432C-A315T^[3].
AIM4 (50 mM stock solution in 25% DMSO; protein:AIM4 1:15; 5 h) inhibits the in vitro liquid-liquid phase separation of recombinant TDP-432C-A315T^[3].
AIM4 (6 mM; 15 h) completely inhibits recombinant TDP-432C aggregation in the ThT fluorescence assay and eliminates the early acceleration of TDP-432C aggregation induced by HSA Protein (HY-P1956)^[4].
AIM4 is a nuclear protein with a functional NLS that may act as a negative regulator of the Slt2 and Hog1 pathways in Saccharomyces cerevisiae, and aim4∆/aim4∆ cells exhibit increased resistance to copper, 1,3,7-trimethylxanthine, and zymolyase^[5].