ALFA-tag
Based on 1 Customer Validation
ALFA-tag is an α-helical peptide epitope tag. ALFA-tag is recognized by the high-affinity Nb-ALFA nanobody (Kd = 26 pM). The ALFA system, composed of ALFA-tag and ALFA-Nb, is widely adopted and applied in various fields such as immunofluorescence, super-resolution microscopy, immunoblotting, and protein purification.
For research use only. We do not sell to patients.
- Purity : 99.40%
- CAS No.: 2413353-86-1
- Formula: C69H123N25O24
- Molecular Weight:1686.87
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
ALFA-tag binds to ALFA-Nb with extremely high affinity (Kd = 26 pM) and has been successfully applied in a variety of extracellular and intracellular contexts[4].
The ALFA system, composed of the ALFA-tag and ALFA-Nb, is widely adopted and applied in various fields such as immunofluorescence, super-resolution microscopy, immunoblotting, and protein purification; meanwhile, it is also the preferred approach for developing "photobodies" targeting short epitope tags[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Western Blot
- Baculovirus-insect cell protein expression and purification
- Antibody-based immunofluorescence/immunocytochemistry staining
- Immunocytochemistry/Immunofluorescence
- Inclusion-body expression, solubilization, refolding and purification
- Protocol For Protein Expression And Purification
- Mammalian transient protein expression and purification
Chemical Information
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CAS No. 2413353-86-1
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Appearance Solid
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Molecular Weight 1686.87
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Formula C69H123N25O24
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Color White to off-white
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Sequence
Ser-Arg-Leu-Glu-Glu-Glu-Leu-Arg-Arg-Arg-Leu-Thr-Glu
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Sequence Shortening
SRLEEELRRRLTE
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 23.81 mg/mL (14.11 mM; adjust pH to 2 with 1 M NaOH)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5928 mL | 2.9641 mL | 5.9281 mL | 14.8203 mL |
| 5 mM | 0.1186 mL | 0.5928 mL | 1.1856 mL | 2.9641 mL | |
| 10 mM | 0.0593 mL | 0.2964 mL | 0.5928 mL | 1.4820 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ALFA-tag Related Classifications
HY-P10531 Related Classifications
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