2413353-86-1
Chemical Structure
ALFA-tag
- CAS No.: 2413353-86-1
- Formula:C69H123N25O24
- Molecular Weight:1686.87
InChIKey: GDPMSDPMZSVCFH-DTGKHZLWSA-N
SMILES: O=C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(N[C@@H]([C@H](O)C)C(N[C@@H](CCC(O)=O)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CO)N
Biological Activity: ALFA-tag is an α-helical peptide epitope tag. ALFA-tag is recognized by the high-affinity Nb-ALFA nanobody (Kd = 26 pM). The ALFA system, composed of ALFA-tag and ALFA-Nb, is widely adopted and applied in various fields such as immunofluorescence, super-resolution microscopy, immunoblotting, and protein purification[1][2][3][4].
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ALFA-tag | 99.40% | ALFA-tag is an α-helical peptide epitope tag. ALFA-tag is recognized by the high-affinity Nb-ALFA nanobody (Kd = 26 pM). The ALFA system, composed of ALFA-tag and ALFA-Nb, is widely adopted and applied in various fields such as immunofluorescence, super-resolution microscopy, immunoblotting, and protein purification. | ||||||||||||||||||||
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References
- [1]. Wang Y, et al. Transport and inhibition mechanism for VMAT2-mediated synaptic vesicle loading of monoamines. Cell research. 2024 Jan;34(1):47-57.
- [2]. Zelek WM, et al. Brain-penetrant complement inhibition mitigates neurodegeneration in an Alzheimer's disease mouse model. Brain : a journal of neurology. 2025 Mar 06;148(3):941-954.
- [3]. Saavedra Pérez Salas G. Generation of a Generic Nanobody-based CAR-T Cell Platform (Doctoral dissertation, et al.
- [4]. Jedlitzke B, et al. A Light-Activatable Photocaged Variant of the Ultra-High Affinity ALFA-Tag Nanobody. Chembiochem : a European journal of chemical biology. 2022 Jun 20;23(12):e202200079.