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Cat. No.: HY-B0067A

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Amrubicin hydrochloride

Name: Amrubicin hydrochloride
Brand: MedChemExpress
SKU: HY-B0067A
Price: 320.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: SM-5887 hydrochloride)

Customer Review

Based on 1 Customer Validation

Amrubicin (SM-5887) hydrochloride is a DNA topoisomerase II inhibitor, used for the research of cancer.

For research use only. We do not sell to patients.

Purity : 98.78%
CAS No.: 110311-30-3
Formula: C₂₅H₂₆ClNO₉
Molecular Weight:519.93

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Amrubicin hydrochloride Chemical Structure

CAS No.: 110311-30-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Amrubicin (SM-5887) hydrochloride is a DNA topoisomerase II inhibitor, used for the research of cancer.

IC₅₀ & Target

^[1]

Topoisomerase II

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.82 μM Compound: amrubicin hydrochloride	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 17970593]
A549	IC₅₀	0.82 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 18578539]
A549	IC₅₀	0.94 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 20681569]
HT-29	IC₅₀	4.36 μM Compound: amrubicin hydrochloride	Cytotoxicity against human HT29 cells after 72 hrs by SRB assay Cytotoxicity against human HT29 cells after 72 hrs by SRB assay	[PMID: 17970593]
HT-29	IC₅₀	4.36 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human HT29 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human HT29 cells after 72 hrs by sulforhodamine B assay	[PMID: 18578539]
HT-29	IC₅₀	4.57 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 20681569]
K562	IC₅₀	1.2 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human K562 cells after 72 hrs by SRB assay Cytotoxicity against human K562 cells after 72 hrs by SRB assay	[PMID: 20681569]
K562	IC₅₀	1.26 μM Compound: amrubicin hydrochloride	Cytotoxicity against human K562 cells after 72 hrs by SRB assay Cytotoxicity against human K562 cells after 72 hrs by SRB assay	[PMID: 17970593]
K562	IC₅₀	1.26 μM Compound: Amrubicin hydrochloride	Cytotoxicity against human K562 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human K562 cells after 72 hrs by sulforhodamine B assay	[PMID: 18578539]

In Vitro

Amrubicin (SM-5887) (2.5 μg/mL) shows radio-enhancement effects on human lung adenocarcinoma A549 cells^[1]. Amrubicin (SM-5887) supresses the LX-1, A549, A431, and BT-474 cell lines, with IC₅₀s of 1.1 ± 0.2, 2.4 ± 0.8, 0.61 ± 0.10 and 3.0 ± 0.3 µg/mL, respectively^[2]. Amrubicin (SM-5887) inhibits the cell cycle profile of U937 cells with an IC₅₀ of 5.6 µM. Amrubicin (20 µM) also induces apoptosis in U937 cells, activates caspase-3/7 and reduces the mitochondrial membrane potential (Δψm)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amrubicin (SM-5887) (25 mg/kg, i.v.) exhibits significant antitumor activities against both SCLC tumors, Lu-24 and Lu-134, with T/C-values (comparing the mean tumor growth rates of the treated group with those of the control group for each day that the tumors are measured) at day 14 of 17% and 9%, respectively. Amrubicin (SM-5887) (25 mg/kg, i.v.) in combination with cisplatin and irinotecan significantly inhibits the growth of tumors compared to amrubicin alone in mice bearing LX-1 tumor cells. Amrubicin (SM-5887) alone or combined with tegafur and uracil also suppresses tumor growth in human cancer xenograft models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Chemical Information

CAS No. 110311-30-3
Appearance Solid
Molecular Weight 519.93
Formula C₂₅H₂₆ClNO₉
Color Brown to red
SMILES

CC([C@@](C1)(N)C[C@H](O[C@@H]2OC[C@@H](O)[C@@H](O)C2)C(C1=C3O)=C(O)C4=C3C(C5=CC=CC=C5C4=O)=O)=O.Cl
Synonyms

SM-5887 hydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (192.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9233 mL

9.6167 mL

19.2334 mL

5 mM

0.3847 mL

1.9233 mL

3.8467 mL

10 mM

0.1923 mL

0.9617 mL

1.9233 mL

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hayashi S, et al. Enhancement of radiosensitivity by topoisomerase II inhibitor, amrubicin and amrubicinol, in human lung adenocarcinoma A549 cells and kinetics of apoptosis and necrosis induction. Int J Mol Med. 2006 Nov;18(5):909-15. [Content Brief]

[2]. Hanada M, et al. Amrubicin, a novel 9-aminoanthracycline, enhances the antitumor activity of chemotherapeutic agents against human cancer cells in vitro and in vivo. Cancer Sci. 2007 Mar;98(3):447-54. [Content Brief]

[3]. Hanada M, et al. Amrubicin induces apoptosis in human tumor cells mediated by the activation of caspase-3/7 preceding a loss of mitochondrial membrane potential. Cancer Sci. 2006 Dec;97(12):1396-403. Epub 2006 Sep 21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9233 mL	9.6167 mL	19.2334 mL	48.0834 mL
	5 mM	0.3847 mL	1.9233 mL	3.8467 mL	9.6167 mL
	10 mM	0.1923 mL	0.9617 mL	1.9233 mL	4.8083 mL
	15 mM	0.1282 mL	0.6411 mL	1.2822 mL	3.2056 mL
	20 mM	0.0962 mL	0.4808 mL	0.9617 mL	2.4042 mL
	25 mM	0.0769 mL	0.3847 mL	0.7693 mL	1.9233 mL
	30 mM	0.0641 mL	0.3206 mL	0.6411 mL	1.6028 mL
	40 mM	0.0481 mL	0.2404 mL	0.4808 mL	1.2021 mL
	50 mM	0.0385 mL	0.1923 mL	0.3847 mL	0.9617 mL
	60 mM	0.0321 mL	0.1603 mL	0.3206 mL	0.8014 mL
	80 mM	0.0240 mL	0.1202 mL	0.2404 mL	0.6010 mL
	100 mM	0.0192 mL	0.0962 mL	0.1923 mL	0.4808 mL

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References

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Amrubicin hydrochloride

All Topoisomerase Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

In Vivo

Clinical Trial

Chemical Information

Solvent & Solubility

In Vitro:

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table