AMT-NHS
Based on 1 Customer Validation
AMT-NHS is an RNA-protein crosslinker. AMT-NHS consists of a psoralen derivative and an N-hydroxysuccinimide ester group, which react with RNA bases and primary amines of proteins, respectively. AMT-NHS penetrates live yeast cells and crosslinks Cbf5 to H/ACA snoRNAs with high specificity. AMT-NHS induces distinct crosslinking patterns and targets both single-stranded and double-stranded regions of RNA. AMT-NHS can be used to capture various RNA-protein interactions in cells.
For research use only. We do not sell to patients.
- Purity : 98.70%
- CAS No.: 2925268-86-4
- Formula: C22H21NO7S
- Molecular Weight:443.47
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
AMT-NHS (0.1-1.5 mM; incubated in the dark for 30 min and irradiated for 30 min) penetrates live Saccharomyces cerevisiae cells and crosslinks Cbf5 to H/ACA snoRNA, and the H/ACA snoRNA readout specificity increases with increasing concentration[1].
AMT-NHS induces significant nonspecific crosslinking of Rnt1 with highly abundant tRNA and rRNA in yeast[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2925268-86-4
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Appearance Solid
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Molecular Weight 443.47
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Formula C22H21NO7S
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Color White to off-white
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SMILES
O=C(ON1C(CCC1=O)=O)CCSCC2=C(C)OC(C(C)=C3O4)=C2C=C3C(C)=CC4=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (56.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2549 mL | 11.2747 mL | 22.5494 mL | 56.3736 mL |
| 5 mM | 0.4510 mL | 2.2549 mL | 4.5099 mL | 11.2747 mL | |
| 10 mM | 0.2255 mL | 1.1275 mL | 2.2549 mL | 5.6374 mL | |
| 15 mM | 0.1503 mL | 0.7516 mL | 1.5033 mL | 3.7582 mL | |
| 20 mM | 0.1127 mL | 0.5637 mL | 1.1275 mL | 2.8187 mL | |
| 25 mM | 0.0902 mL | 0.4510 mL | 0.9020 mL | 2.2549 mL | |
| 30 mM | 0.0752 mL | 0.3758 mL | 0.7516 mL | 1.8791 mL | |
| 40 mM | 0.0564 mL | 0.2819 mL | 0.5637 mL | 1.4093 mL | |
| 50 mM | 0.0451 mL | 0.2255 mL | 0.4510 mL | 1.1275 mL |
AMT-NHS Related Classifications
HY-150014 Related Classifications
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