Cat. No.: HY-183934

Anti-inflammatory agent 99

Anti-inflammatory agent 99 is a chalcone derivative. Anti-inflammatory agent 99 inhibits LPS (HY-D1056)-induced NF-κB nuclear translocation and suppress the phosphorylation of JNK, ERK, and p38. Anti-inflammatory agent 99 inhibits the expression of cytoinflammatory factors such as TNF-α and IL-6 induced by LPS. Anti-inflammatory agent 99 can be used for the research of LPS-induced septic shock.

For research use only. We do not sell to patients.

CAS No.: 1345412-48-7
Formula: C₁₆H₁₂F₂O₂
Molecular Weight:274.26

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Anti-inflammatory agent 99 Chemical Structure

CAS No.: 1345412-48-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

JNK

NF-κB

p38

ERK

IL-6

All NF-κB Isoforms :

All JNK Isoforms :

All ERK Isoforms :

All p38 MAPK Isoforms :

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	5.55 μM Compound: 26	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA	[PMID: 21988173]
RAW264.7	IC₅₀	8.42 μM Compound: 26	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production incubated for 2 hrs prior to LPS-challenge measured after 22 hrs by ELISA	[PMID: 21988173]

In Vitro

Anti-inflammatory agent 99 (Compound 26) (2.5-10 μM; 24 h) dose-dependently inhibits LPS-induced TNF-α and IL-6 release in mouse RAW 264.7 macrophages, with IC₅₀ values of 8.42 μM and 5.55 μM, respectively^[1].
Anti-inflammatory agent 99 (10 μM; 8 h) inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression in mouse primary peritoneal macrophages, but does not significantly reduce COX-2 mRNA levels^[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5-3 h) inhibits LPS-induced NF-κB p65 nuclear translocation, and dose-dependently prevents LPS-induced IκB degradation in mouse primary peritoneal macrophages^[1].
Anti-inflammatory agent 99 (2.5-20 μM; 2.5 h) dose-dependently inhibits LPS-induced JNK phosphorylation in mouse primary peritoneal macrophages^[1].
Anti-inflammatory agent 99 (2.5-20 μM; 3 h) inhibits LPS-induced ERK and p38 phosphorylation, and dose-dependently blocks LPS-induced p38 and ERK activation in mouse primary peritoneal macrophages^[1].
Anti-inflammatory agent 99 (10 μM; 5 h) inhibits high Glucose (HY-B0389)-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression in mouse primary peritoneal macrophages^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

mouse RAW 264.7 macrophages
Concentration:

2.5 μM; 5.0 μM; 10 μM
Incubation Time:

24 h
Result:

Dose-dependently inhibited LPS-induced TNF-α and IL-6 release.
Exhibited an IC₅₀ of 8.42 μM for TNF-α inhibition and an IC₅₀ of 5.55 μM for IL-6 inhibition.
Significantly reduced TNF-α and IL-6 levels compared to the LPS-only group at 10 μM (p < 0.01).

Real Time qPCR^[1]

Cell Line:

mouse primary peritoneal macrophages
Concentration:

10 μM
Incubation Time:

8 h
Result:

Inhibits LPS-induced TNF-α, IL-6, IL-1β, and IL-12 mRNA expression.
Did not significantly reduce COX-2 mRNA levels.

Immunofluorescence^[1]

Cell Line:

mouse primary peritoneal macrophages
Concentration:

10 μM
Incubation Time:

2.5 h
Result:

Inhibited LPS-induced NF-κB p65 nuclear translocation.

Western Blot Analysis^[1]

Cell Line:

mouse primary peritoneal macrophages
Concentration:

2.5 μM; 5 μM; 10 μM; 20 μM
Incubation Time:

3 h
Result:

Dose-dependently inhibited LPS-induced IκB degradation, JNK phosphorylation and activation of p38 and ERK.
Inhibited LPS-induced ERK and p38 phosphorylation at 10 μM.

Real Time qPCR^[1]

Cell Line:

mouse primary peritoneal macrophages
Concentration:

10 μM
Incubation Time:

5 h
Result:

Inhibited high Glucose-induced TNF-α, IL-1β, IL-6, and IL-12 mRNA expression.

In Vivo

Anti-inflammatory agent 99 (Compound 26) (15 mg/kg; i.v.; single dose) confers an 80% survival rate in mice with LPS-induced inflammatory shock, significantly reducing LPS-induced lethality^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (B6) (male, 18−22 g, LPS-induced inflammatory shock model)^[1]
Dosage:

15 mg/kg
Administration:

i.v.; single dose
Result:

Exhibited an 80% survival rate over the 7-day observation period.
Showed body weight decrease during the first 60 hours after LPS treatment that regained slowly afterward.

Related Protocols :

Chemical Information

CAS No. 1345412-48-7
Molecular Weight 274.26
Formula C₁₆H₁₂F₂O₂
SMILES

O=C(C1=CC=C(C=C1)OC)/C=C/C2=C(C=CC=C2F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (364.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6462 mL

18.2309 mL

36.4618 mL

5 mM

0.7292 mL

3.6462 mL

7.2923 mL

10 mM

0.3646 mL

1.8231 mL

3.6462 mL

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Wu J, et al. Evaluation and discovery of novel synthetic chalcone derivatives as anti-inflammatory agents. J Med Chem. 2011;54(23):8110-8123. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6462 mL	18.2309 mL	36.4618 mL	91.1544 mL
	5 mM	0.7292 mL	3.6462 mL	7.2924 mL	18.2309 mL
	10 mM	0.3646 mL	1.8231 mL	3.6462 mL	9.1154 mL
	15 mM	0.2431 mL	1.2154 mL	2.4308 mL	6.0770 mL
	20 mM	0.1823 mL	0.9115 mL	1.8231 mL	4.5577 mL
	25 mM	0.1458 mL	0.7292 mL	1.4585 mL	3.6462 mL
	30 mM	0.1215 mL	0.6077 mL	1.2154 mL	3.0385 mL
	40 mM	0.0912 mL	0.4558 mL	0.9115 mL	2.2789 mL
	50 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8231 mL
	60 mM	0.0608 mL	0.3038 mL	0.6077 mL	1.5192 mL
	80 mM	0.0456 mL	0.2279 mL	0.4558 mL	1.1394 mL
	100 mM	0.0365 mL	0.1823 mL	0.3646 mL	0.9115 mL

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Anti-inflammatory agent 99

All NF-κB Isoforms

All JNK Isoforms

All ERK Isoforms

All p38 MAPK Isoforms

All TNF Receptor Isoforms

All Interleukin Related Isoforms