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ARF4 Antibody

(Synonyms: ARF2, ARF4, ADP-ribosylation factor 4)
Customer Review

Based on 1 Customer Validation

ARF4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARF4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ARF4 Antibody
  • ARF4 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1000 1:50-100 1:50-200

Product Details

Description

ARF4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARF4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 20 kDa
Immunogen

Synthetic peptide corresponding to the center region of human ARF4.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ARF4 Antibody
    Western blot analysis of extracts from HT-29 (lane 2, 20ug) and K562 (lane 3, 20ug) using ARF4 Antibody (HY-P811015). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 2 hour at room temperature.

Background

  • Function

    ARF4 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation[1].

  • Subcellular Localization

    Golgi apparatus; Membrane

  • Isoforms & Post-Translational Modification

    ARF4 has an amino acid length of 180, molecular weight is 20511 Da.

  • Subunit

    Forms a complex containing RAB11A, ASAP1, RAB3IP, RAP11FIP3 and ARF4; the complex promotes preciliary trafficking; the complex binds to RHO in photoreceptor cells and promotes RHO ciliary transport

  • SwissProt ID

    P18085

  • Gene ID
    378 [NCBI]
  • Synonyms

    ARF2, ARF4, ADP-ribosylation factor 4

References

[1]. Wang J, et al. The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to direct Rabin8 in ciliary receptor targeting. J Cell Sci. 2015 Apr 1;128(7):1375-85. [Content Brief]

ARF4 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2, 20ug) and K562 (lane 3, 20ug) using ARF4 Antibody (HY-P811015). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 2 hour at room temperature.

  • ARF4 Antibody Product Image
    1/16
  • Experimental Validation Results for ARF4 Antibody
  • ARF4 Antibody Product Image
  • Experimental Validation Results for ARF4 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2, 20ug) and K562 (lane 3, 20ug) using ARF4 Antibody (HY-P811015). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 2 hour at room temperature.
  • Experimental Validation Results for ARF4 Antibody
  • ARF4 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29 (lane 2, 20ug) and K562 (lane 3, 20ug) using ARF4 Antibody (HY-P811015). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 2 hour at room temperature.

  • ARF4 Antibody Product Image WB