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Cripto1/CRIPTO Antibody (YA2245)

(Synonyms: CRGF; Cripto 1; cripto; Cripto1 growth factor; TDGF1)
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Based on 1 Customer Validation

Cripto1/CRIPTO Antibody (YA2245) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cripto1/CRIPTO.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Cripto1/CRIPTO Antibody (YA2245) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50 1:50-1:100

Product Details

Description

Cripto1/CRIPTO Antibody (YA2245) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cripto1/CRIPTO.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database

Entrez Gene: 6997 Human

SwissProt: P13385 Human

Immunogen

A synthesized peptide derived from human Cripto1 aa20-88.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    Western blot analysis was performed on extracts from NCCIT (lane 1, 15 μg) using Cripto 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cripto1/CRIPTO is a GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm[1][2].

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Secreted

  • Expression


    Tissue_specificity:This gene is preferentially expressed in gastric and colorectal cancers compared to normal tissues. It is also expressed in breast and lung cancers.

  • Subunit

    Interacts with the activin type-1 receptor ACVR1B

  • SwissProt ID

    P13385

  • Gene ID
    6997 [NCBI]
  • Synonyms

    CRGF; Cripto 1; cripto; Cripto1 growth factor; TDGF1

  • Research Field

    Stem Cells

Cripto1/CRIPTO Antibody (YA2245) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from NCCIT (lane 1, 15 μg) using Cripto 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cripto1/CRIPTO Antibody (YA2245) Product Image
    1/16
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Cripto1/CRIPTO Antibody (YA2245) Product Image
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from NCCIT (lane 1, 15 μg) using Cripto 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Experimental Validation Results for Cripto1/CRIPTO Antibody (YA2245)
  • Cripto1/CRIPTO Antibody (YA2245) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from NCCIT (lane 1, 15 μg) using Cripto 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cripto1/CRIPTO Antibody (YA2245) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cripto1/CRIPTO Antibody (YA2245) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Cripto1/CRIPTO with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cripto1/CRIPTO Antibody (HY-P82500) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cripto1/CRIPTO Antibody (YA2245) Product Image WB
  • Cripto1/CRIPTO Antibody (YA2245) Product Image ICC
  • Cripto1/CRIPTO Antibody (YA2245) Product Image ICC