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Glutaredoxin Antibody

(Synonyms: GRX, GLRX, Glutaredoxin-1, Thioltransferase-1, TTase-1)

Glutaredoxin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Glutaredoxin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glutaredoxin Antibody
  • Glutaredoxin Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-200 1:50-200

Product Details

Description

Glutaredoxin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Glutaredoxin.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 12 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Immunogen

Purified recombinant protein of human Glutaredoxin

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glutaredoxin Antibody
    Western blot analysis of extracts from A549 (lane2, 20μg), Jurkat (lane3, 20μg), Mouse lung tissue (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using Glutaredoxin Antibody (HY-P811248). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Glutaredoxin is a Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Glutaredoxin has an amino acid length of 106, molecular weight is 11776 Da.

  • SwissProt ID

    P35754

  • Gene ID
    2745 [NCBI]
  • Synonyms

    GRX, GLRX, Glutaredoxin-1, Thioltransferase-1, TTase-1

Glutaredoxin Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), Jurkat (lane3, 20μg), Mouse lung tissue (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using Glutaredoxin Antibody (HY-P811248). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Glutaredoxin Antibody Product Image
    1/16
  • Experimental Validation Results for Glutaredoxin Antibody
  • Glutaredoxin Antibody Product Image
  • Experimental Validation Results for Glutaredoxin Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), Jurkat (lane3, 20μg), Mouse lung tissue (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using Glutaredoxin Antibody (HY-P811248). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glutaredoxin Antibody
  • Glutaredoxin Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane2, 20μg), Jurkat (lane3, 20μg), Mouse lung tissue (lane4, 20μg) and Mouse liver tissue (lane5, 20μg) using Glutaredoxin Antibody (HY-P811248). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Glutaredoxin Antibody Product Image WB