MRPL12 Antibody
(Synonyms: MRPL7; RPML12; MRPL12; Large ribosomal subunit protein bL12m; 5c5-2; L12mt; MRP-L12)MRPL12 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRPL12.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
MRPL12 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRPL12.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 21 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 21 kDa
Synthetic peptide corresponding to the center region of human MRPL12.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg) using MRPL12 Antibody (HY-P810920). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
MRPL12 is an As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability[1][2][3].
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Subcellular Localization
Mitochondrion matrix
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Isoforms & Post-Translational Modification
MRPL12 has an amino acid length of 198, molecular weight is 21348 Da.
Two mature forms are produced by differential two-step proteolytic cleavage. Cleaved by the mitochondrial processing protease to produce the long mature form and subsequently by the mitochondrial intermediate protease to produce the short mature form. -
Subunit
Component of the mitochondrial large ribosomal subunit (mt-LSU).
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SwissProt ID
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Synonyms
MRPL7; RPML12; MRPL12; Large ribosomal subunit protein bL12m; 5c5-2; L12mt; MRP-L12
Documentation
References
[1]. Serre V, et al. Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency. Biochim Biophys Acta. 2013 Aug;1832(8):1304-12. [Content Brief]
[2]. Surovtseva YV, et al. Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription. Proc Natl Acad Sci U S A. 2011 Nov 1;108(44):17921-6. [Content Brief]
[3]. Nouws J, et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem. 2016 Jan 8;291(2):989-97. [Content Brief]
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