Antimicrobial peptide BP100 TFA
Based on 1 Customer Validation
Antimicrobial peptide BP100 TFA is a linear undecapeptide with an amphipathic α-helical structure that exhibits antibacterial activity. Antimicrobial peptide BP100 TFA shows in vitro antibacterial activity against a variety of plant and human pathogens. Antimicrobial peptide BP100 TFA binds to and inserts into bacterial cell membranes through electrostatic attraction, triggering membrane permeabilization and ultimately leading to membrane disruption. Antimicrobial peptide BP100 TFA can be used for research on bacterial infections.
For research use only. We do not sell to patients.
- Purity : 98.25%
- Formula: C72H125N17O12·xC2HF3O2
- Molecular Weight:1420.87 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Antimicrobial peptide BP100 (0.3-8 μM; 0.5-5 h) TFA disrupts the cell envelope, causes membrane surface blebbing and collapse, and induces leakage of intracellular contents in E. coli (ATCC 25922) cells[4].
Antimicrobial peptide BP100 (0.5-8 μM; 15 min) TFA neutralizes the negative charge on the membrane surface and alters the Zeta potential in E. coli cells, with the concentration for complete neutralization being approximately 2-4 µM[4].
Antimicrobial peptide BP100 (0.8-50 μM; 48 h) TFA exhibits antibacterial activity by inhibiting bacterial growth in plant pathogenic cells such as E. amylovora and P. syringae[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1420.87 (free base)
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Formula C72H125N17O12·xC2HF3O2
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Color White to off-white
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SMILES
O=C(N[C@@H](CCCCN)C(N[C@@H](CC(C)C)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CC(C)C)C(N[C@@H](CCCCN)C(N[C@@H](CC2=CC=C(C=C2)O)C(N[C@@H](CC(C)C)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CCCCN)N.OC(C(F)(F)F)=O.[x]
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Sequence
Lys-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Tyr-Leu-NH2
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Sequence Shortening
KKLFKKILKYL-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : ≥ 50 mg/mL
* "≥" means soluble, but saturation unknown.
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antimicrobial peptide BP100 TFA Related Classifications
HY-P12210A Related Classifications
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Keywords
- Antimicrobial peptide BP100
- Antimicrobial peptide BP 100
- Antimicrobial peptide BP-100
- Bacterial
- Drug Intermediate
- Erwinia amylovora
- Xanthomonas axonopodis
- Dickeya chrysanthemi
- Pseudomonas syringae
- Nicotiana benthamiana
- colistin-resistant Acinetobacter baumannii
- Bacillus subtilis
- E. coli (ATCC 25922)
- HeLa cells
- Fusarium verticillioides
- Inhibitor
- inhibitor
- inhibit