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Cat. No.: HY-D2047

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ATTO 532 maleimide

ATTO 532 maleimide is a thiol-reactive Fluorescent dye. ATTO 532 maleimide enables site-specific labeling of proteins via maleimide-cysteine chemistry and can be used for single-molecule detection of GroEL EL315C binding/complex formation and single-molecule FRET, and can label surface cysteine substrates, Prothymosin α, Frataxin, and GB1. ATTO 532 maleimide covalently labels Tau, serves as a fluorescence lifetime sensor of amyloid aggregate structural density, and exhibits self-quenching.

For research use only. We do not sell to patients.

CAS No.: 1632109-97-7
Formula: C₃₃H₃₂N₄O₁₂S₂
Molecular Weight:740.76

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (275 KB)

ATTO 532 maleimide Chemical Structure

CAS No.: 1632109-97-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
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References
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Biological Activity

Description

In Vitro

Protein/Amino Acid MetabolismOthers

Protein/Amino Acid Metabolism

ATTO-532 maleimide (100 μM; 2 h) labels purified poliovirus Ub-peptide-Ub and Ub-3aBC substrates with 50 μM protein, enabling fluorescence detection of 3Cpro- and 3ABCpro-mediated cleavage; in these assays 3ABCpro is activated 24-fold by cre26 RNA and 10-fold by polyA15 RNA, whereas 3Cpro is activated only 2-fold by cre26 RNA^[5].

Others

ATTO 532 maleimide (1:6.5 molar ratio to EL315C monomer; 45 min) conjugates to GroEL EL315C, yielding fluorescently labeled GroEL-ATTO532^[1].
ATTO 532 maleimide (10 mM; 2 h) labels recombinant human vimentin monomers via maleimide chemistry, yielding labeled protein at 4%-41% labeling ratios^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Amyloid: Congo Red Amyloid Staining

Chemical Information

CAS No. 1632109-97-7
Molecular Weight 740.76
Formula C₃₃H₃₂N₄O₁₂S₂
SMILES

O=C(CCCN(C)C(C1=C(C2=C(C=CC(NCC)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(NCC)=C4S(=O)([O-])=O)C=CC=C1)=O)ON5C(C=CC5=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Naqvi MM, et al. Protein chain collapse modulation and folding stimulation by GroEL-ES. Science advances. 2022 Mar 04;8(9):eabl6293.

[2]. König I, et al. Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells. Nature methods. 2015 Aug;12(8):773-9.

[3]. Perego E, et al. Exploring early time points of vimentin assembly in flow by fluorescence fluctuation spectroscopy. Lab on a Chip. 2021 Feb 21;21(4):735-45.

[4]. Chen W, et al. Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells. Nano letters. 2017 Jan 11;17(1):143-149.

[5]. Campagnola G, et al. Co-folding and RNA activation of poliovirus 3C polyprotein precursors. The Journal of biological chemistry. 2023 Nov;299(11):105258.

[6]. Majumder S, et al. Hotspot site microenvironment in the deubiquitinase OTUB1 drives its stability and aggregation. The Journal of biological chemistry. 2024 Jun;300(6):107315.

[7]. Subekti DR. Dynamics of Tumor Suppressor p53 along DNA Revealed by High-speed Single-molecule Fluorescence Microscopy (Doctoral dissertation, et al.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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