1632109-97-7
Chemical Structure
ATTO 532 maleimide
- CAS No.: 1632109-97-7
- Formula:C33H32N4O12S2
- Molecular Weight:740.76
InChIKey: JPIMDLNMFCTYFI-UHFFFAOYSA-N
SMILES: O=C(CCCN(C)C(C1=C(C2=C(C=CC(NCC)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(NCC)=C4S(=O)([O-])=O)C=CC=C1)=O)ON5C(C=CC5=O)=O
Biological Activity: ATTO 532 maleimide is a thiol-reactive Fluorescent dye. ATTO 532 maleimide enables site-specific labeling of proteins via maleimide-cysteine chemistry and can be used for single-molecule detection of GroEL EL315C binding/complex formation and single-molecule FRET, and can label surface cysteine substrates, Prothymosin α, Frataxin, and GB1. ATTO 532 maleimide covalently labels Tau, serves as a fluorescence lifetime sensor of amyloid aggregate structural density, and exhibits self-quenching[1][2][3][4][5][6][7].
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ATTO 532 maleimide | ATTO 532 maleimide is a thiol-reactive Fluorescent dye. ATTO 532 maleimide enables site-specific labeling of proteins via maleimide-cysteine chemistry and can be used for single-molecule detection of GroEL EL315C binding/complex formation and single-molecule FRET, and can label surface cysteine substrates, Prothymosin α, Frataxin, and GB1. ATTO 532 maleimide covalently labels Tau, serves as a fluorescence lifetime sensor of amyloid aggregate structural density, and exhibits self-quenching. | |||||||||||||||||||||
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References
- [1]. Naqvi MM, et al. Protein chain collapse modulation and folding stimulation by GroEL-ES. Science advances. 2022 Mar 04;8(9):eabl6293.
- [2]. König I, et al. Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells. Nature methods. 2015 Aug;12(8):773-9.
- [3]. Perego E, et al. Exploring early time points of vimentin assembly in flow by fluorescence fluctuation spectroscopy. Lab on a Chip. 2021 Feb 21;21(4):735-45.
- [4]. Chen W, et al. Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells. Nano letters. 2017 Jan 11;17(1):143-149.
- [5]. Campagnola G, et al. Co-folding and RNA activation of poliovirus 3C polyprotein precursors. The Journal of biological chemistry. 2023 Nov;299(11):105258.
- [6]. Majumder S, et al. Hotspot site microenvironment in the deubiquitinase OTUB1 drives its stability and aggregation. The Journal of biological chemistry. 2024 Jun;300(6):107315.
- [7]. Subekti DR. Dynamics of Tumor Suppressor p53 along DNA Revealed by High-speed Single-molecule Fluorescence Microscopy (Doctoral dissertation, et al.
