D-Ribose-L-cysteine
Based on 1 Customer Validation
D-Ribose-L-cysteine is an orally effective glutathione (GSH) precursor that crosses the blood-brain barrier. D-Ribose-L-cysteine exhibits potent antioxidant, anti-inflammatory, and neuro- and organ-protective activities. D-Ribose-L-cysteine exerts dual antioxidant and anti-inflammatory effects by promoting endogenous GSH synthesis to scavenge reactive oxygen species (ROS) and inhibiting NF-κB and the expression of its downstream pro-inflammatory factors (TNF-α, IL-6), thereby inhibiting apoptosis. D-Ribose-L-cysteine also reverses Scopolamine (HY-N0296)-induced memory impairment by inhibiting acetylcholinesterase (AChE) activity. D-Ribose-L-cysteine can be used in research on Alzheimer's disease, cardiometabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease.
For research use only. We do not sell to patients.
- Purity : 99.79%
- CAS No.: 232617-15-1
- Formula: C8H15NO6S
- Molecular Weight:253.27
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Swiss (male, 20-25 g)[1]
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Dosage:25, 50, 100 mg/kg
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Administration:p.o.; once daily; 28 days
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Result:Reversed memory impairment and dysregulated levels of malondialdehyde, glutathione, nitrite, and glutathione S-transferase in liver and brain tissues.
Reduced elevated proinflammatory cytokines, brain AChE, and liver enzyme activities.
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Animal Model:Wistar (male, 200-220 g)[2]
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Dosage:250 mg/kg
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Administration:p.o.; once daily; 8 weeks
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Result:Prevented the manifestation of oxidative stress and cardiometabolic syndrome, normalized lipid and glucose homeostasis, and reduced cardiac xanthine oxidase and systolic blood pressure.
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Animal Model:Wistar (adult albino male, 150-200 g)[4]
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Dosage:200 mg/kg
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Administration:p.o.; once daily; 14 days
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Result:Improved glutathione levels, attenuated neuronal and mitochondrial ultrastructural damage, and reduced caspase-3 and GFAP expressions.
Mitigated cognitive and motor deficits, oxidative damage, and reactive microglia activation.
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Animal Model:Swiss (male; 20-25 g; 8 weeks old)[7]
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Dosage:25, 50, 100 mg/kg
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Administration:p.o.; once daily; 7 days
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Result:Significantly enhanced memory performance, attenuated scopolamine-induced amnesia, and decreased acetylcholinesterase activity and oxidative stress.
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Animal Model:Swiss (male, 22 g, 7-8 weeks old)[8]
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Dosage:25, 50, 100 mg/kg
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Administration:p.o.; once daily; for 7 consecutive days
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Result:Reversed memory deficits, reduced oxidative stress and proinflammatory cytokines, decreased AChE activity and NF-κB expression, and attenuated cytoarchitectural distortions in the brain.
- RNA extraction experimental
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Apoptosis Solutions
- Research Protocol for Cardiovascular Diseases
- Research Protocol for Inflammation-related Diseases
- Research Protocol for Metabolic Diseases
- Alzheimer’s Disease Modeling
Chemical Information
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CAS No. 232617-15-1
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Appearance Solid-Liquid Mixture
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Molecular Weight 253.27
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Formula C8H15NO6S
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Color Light yellow to yellow
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SMILES
O=C(O)[C@H]1NC(SC1)[C@@H]([C@@H]([C@@H](CO)O)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (197.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (295 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9484 mL | 19.7418 mL | 39.4836 mL | 98.7089 mL |
| 5 mM | 0.7897 mL | 3.9484 mL | 7.8967 mL | 19.7418 mL | |
| 10 mM | 0.3948 mL | 1.9742 mL | 3.9484 mL | 9.8709 mL | |
| 15 mM | 0.2632 mL | 1.3161 mL | 2.6322 mL | 6.5806 mL | |
| 20 mM | 0.1974 mL | 0.9871 mL | 1.9742 mL | 4.9354 mL | |
| 25 mM | 0.1579 mL | 0.7897 mL | 1.5793 mL | 3.9484 mL | |
| 30 mM | 0.1316 mL | 0.6581 mL | 1.3161 mL | 3.2903 mL | |
| 40 mM | 0.0987 mL | 0.4935 mL | 0.9871 mL | 2.4677 mL | |
| 50 mM | 0.0790 mL | 0.3948 mL | 0.7897 mL | 1.9742 mL | |
| 60 mM | 0.0658 mL | 0.3290 mL | 0.6581 mL | 1.6451 mL | |
| 80 mM | 0.0494 mL | 0.2468 mL | 0.4935 mL | 1.2339 mL | |
| 100 mM | 0.0395 mL | 0.1974 mL | 0.3948 mL | 0.9871 mL |
D-Ribose-L-cysteine Related Classifications
HY-118642 Related Classifications
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Keywords
- D-Ribose-L-cysteine
- 232617-15-1
- Cholinesterase (ChE)
- Reactive Oxygen Species (ROS)
- NF-κB
- TNF Receptor
- Apoptosis
- Interleukin Related
- ERK
- acetylcholinesterase
- antioxidant
- apoptosis
- blood-brain barrier
- glutathione precursor
- lipid peroxidation
- neuroinflammation
- nitrosative stress
- oxidative stress
- pro-inflammatory cytokines
- Inhibitor
- inhibitor
- inhibit