Diethylumbelliferyl phosphate
Based on 1 Customer Validation
Diethylumbelliferyl phosphate (DEUP) is a broad-spectrum inhibitor of serine esterase and lipases. Diethylumbelliferyl phosphate inhibits cholesteryl ester hydrolase (CEH), adipose triglyceride lipase (ATGL/cytosolic lipase), and retinyl ester hydrolase. Diethylumbelliferyl phosphate blocks the hydrolysis of triglycerides and cholesteryl esters by inhibiting key hydrolases such as CEH and ATGL, thereby inhibiting the mobilization and remodeling of lipid droplet contents, which in turn reduces intracellular reactive oxygen species (ROS) levels and inhibits apoptosis. Diethylumbelliferyl phosphate can be used in research on lipid and cholesterol metabolic diseases and Alzheimer's disease.
For research use only. We do not sell to patients.
- Purity : 99.2%
- CAS No.: 897-83-6
- Formula: C14H17O6P
- Molecular Weight:312.25
-
Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
Diethylumbelliferyl phosphate (800 μM; 1 h pretreatment and subsequent co-incubation for 4-14 h) significantly reduces the triglyceride hydrolysis rate in 3T3-L1 fibroblasts/adipocytes, but does not affect lipid droplet dispersion induced by Forskolin (HY-15371) and IBMX (HY-12318)[2].
Diethylumbelliferyl phosphate (1500 μM; 24 h) in rat primary hepatocytes cultured in a Collagen (HY-NP003) sandwich causes lipid droplet accumulation, upregulates PLN2 and PLN5 expression, reduces intracellular free fatty acid and ATP levels, and disrupts polarized formation of the bile canalicular network[3].
Diethylumbelliferyl phosphate (100 μM) inhibits lipolysis and increases cellular triglyceride accumulation in cultured hepatocytes (RALA255-10G)[4].
Diethylumbelliferyl phosphate (2 h) significantly inhibits progesterone production induced by LH (HY-P2293) and FGIN-1-27 (HY-101059) in MA-10 cells[5].
Diethylumbelliferyl phosphate (100 μM) causes developmental arrest, significantly reduces the total number of lipid droplets, and increases the proportion of large lipid droplets in BTBR mouse preimplantation embryos[7].
Diethylumbelliferyl phosphate (0.1-10 μM; 6 h) inhibits Dibutyryl-cAMP (HY-B0764)-stimulated progesterone synthesis in MA-10 cells in a dose-dependent manner, with half-maximal inhibition at 1 μM and nearly complete inhibition at 10 μM[10].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:3T3-L1 fibroblasts stably expressing ectopic perilipin A and control 3T3-L1 fibroblasts
-
Concentration:800 μM; 10 μM Forskolin; 0.5 mM IBMX; 200 μM oleate (lipid loading)
-
Incubation Time:1h pre-treatment and subsequent tracking at 4, 8, 12, 14 h
-
Result:Significantly decreased the rate of triglyceride hydrolysis under both basal and stimulated conditions, but failed to prevent the Forskolin- and IBMX-induced dispersion of lipid droplets and phosphorylation of perilipin A.
-
Cell Line:MA-10 cells
-
Concentration:0.1, 5, 10 μM (combined with 1 mM Dibutyryl-cAMP)
-
Incubation Time:6 h
-
Result:Blocked the appearance and accumulation of Dibutyryl-cAMP-induced 30-kDa StAR proteins in the mitochondria in a dose-dependent manner.
Chemical Information
-
CAS No. 897-83-6
-
Appearance Liquid (Density: 1.272 g/cm3)
-
Molecular Weight 312.25
-
Formula C14H17O6P
-
Color Colorless to light yellow
-
SMILES
O=C1OC2=C(C(C)=C1)C=CC(OP(OCC)(OCC)=O)=C2
-
Synonyms
DEUP
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
-
Data Sheet (288 KB)
-
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
-
Handling Instructions (2659 KB)
References
[3]. Kang SWS, et al. Multiple cellular pathways regulate lipid droplet homeostasis for the establishment of polarity in collagen sandwich-cultured hepatocytes. American journal of physiology. Cell physiology. 2019 Nov 01;317(5):C942-C952. [Content Brief]
[4]. Singh R, et al. Autophagy regulates lipid metabolism. Nature. 2009 Apr 30;458(7242):1131-5. [Content Brief]
[5]. Chung JY, et al. Cholesterol accumulation, lipid droplet formation, and steroid production in Leydig cells: Role of translocator protein (18-kDa). Andrology. 2020 May;8(3):719-730. [Content Brief]
[7]. Żbikowska D, et al. Strain- and lineage-specific regulation of lipid droplet metabolism in the mouse blastocyst†. Biology of reproduction. 2026 Aug 18;115(2):469-482. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Diethylumbelliferyl phosphate Related Classifications
HY-134451 Related Classifications
Powered by Bioz
Keywords
- Diethylumbelliferyl
- 897-83-6
- DEUP
- ATGL
- Lipase
- Hormone-Sensitive Lipase (HSL)
- Reactive Oxygen Species (ROS)
- Apoptosis
- amyloid-beta degradation
- cholesterol ester hydrolase
- cytosolic lipases
- folic-acid kidney injury
- hepatocyte polarization
- lipid droplet mobilization
- lipolysis
- progesterone production
- triacylglycerol hydrolysis
- Inhibitor
- inhibitor
- inhibit