Erythrosine B aluminum
Erythrosine B aluminum (Erythrosin extra bluish aluminum; Tetraiodofluorescein aluminum) is a visible red dye and insecticide with colorimetric and fluorescent properties. Erythrosine B aluminum serves as a membrane-impermeant live-cell dye for Gram-positive and Gram-negative bacteria, primarily staining dead cells with damaged plasma membranes. Erythrosine B aluminum exhibits photosensitive larvicidal toxicity against Culex pipiens quinquefasciatus larvae. Erythrosine B aluminum is used in live/dead bacterial assays in colorimetric and fluorescent measurements for low-, medium-, and high-throughput experiments, as well as in research related to mosquito-borne diseases and food coloring.
For research use only. We do not sell to patients.
- CAS No.: 12227-78-0
- Formula: C20H8I4O5.2/3Al
- Molecular Weight:853.88
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Erythrosine B aluminum shows a linear correlation between its absorbance at 530 nm and the proportion of dead cells in E. coli, K. pneumoniae, and P. mirabilis populations, enabling microplate-based quantitative colorimetric viability detection[2].
Erythrosine B (5 min) aluminum is a fluorescent viability dye detectable by flow cytometry that distinguishes live from dead cells across multiple bacterial species and tracks the time-dependent increase in cell death in sodium azide-treated cultures of S. marcescens[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Erythrosine B (0.25-4 ppm; added to rearing water for larval ingestion; 24-h dark feeding period prior to light exposure; light exposure duration up to 24 h) aluminum exhibits approximately 16-fold greater larvicidal efficacy against fourth-instar Culex pipiens quinquefasciatus compared with conditions without dark pre-incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:fourth-instar larvae (laboratory colony)[3]
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Dosage:0.25 ppm, 0.5 ppm, 1 ppm, 2 ppm, 4 ppm, 8 ppm, 12 ppm
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Administration:added to rearing water for larval ingestion; ~15 min feeding before light exposure; up to 24 h light exposure
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Result:Showed LC50 values of 9.05 ppm at 3 h, 7.81 ppm at 4 h, 6.61 ppm at 5 h, 5.89 ppm at 6 h, 5.44 ppm at 7 h, and 2.45 ppm at 24 h of light exposure.
Exhibited LT50 values of 7.76 h at 4 ppm, 6.73 h at 8 ppm, and 2.50 h at 12 ppm.
Demonstrated greater toxicity and faster action than fluorescein and both 1:1 and 1:3 nondispersible coprecipitated combinations.
When 8% SDS was added, the results showed LC50 values of 6.86 ppm at 0.5 h, 3.89 ppm at 1 h, 2.52 ppm at 2 h, 2.06 ppm at 3 h, 1.84 ppm at 4 h, 1.66 ppm at 5 h, 1.59 ppm at 6 h, 1.48 ppm at 7 h, and 0.66 ppm at 24 h of light exposure; LT50 values of 25.05 h at 0.5 ppm, 13.87 h at 1 ppm, 5.65 h at 2 ppm, 2.15 h at 4 ppm, 1.02 h at 5 ppm, and 0.49 h at 6 ppm; and approximately 3-fold greater toxicity than the nondispersible formulation after 24 h of light exposure.
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Animal Model:fourth-instar larvae (laboratory colony)[3]
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Dosage:0.25 ppm, 0.50 ppm, 1 ppm, 2 ppm, 4 ppm
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Administration:added to rearing water for larval ingestion; 24-h dark feeding period before light exposure; up to 24 h light exposure
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Result:Showed LC50 values of 1.69 ppm at 0.75 h, 1.19 ppm at 1 h, 0.27 ppm at 2 h, 0.20 ppm at 3 h, and 0.14 ppm at 4 h of light exposure.
Exhibited LT50 values of 2.54 h at 0.25 ppm, 1.95 h at 0.50 ppm, 1.08 h at 1 ppm, 0.76 h at 2 ppm, and 0.66 h at 4 ppm.
Increased toxic effect by about 16-fold relative to the dispersible formulation without dark preincubation.
Shortened the time required to kill 50% of test larvae.
Chemical Information
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CAS No. 12227-78-0
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Molecular Weight 853.88
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Formula C20H8I4O5.2/3Al
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(I)C(O)=C4I)C(I)=C(O)C(I)=C3)C5=C1C=CC=C5.[2/3].[Al]
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Synonyms
Erythrosin extra bluish aluminum; Tetraiodofluorescein aluminum
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Erythrosine B aluminum Related Classifications
HY-107864A Related Classifications
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