Erythrosine B free acid
Based on 1 Customer Validation
Erythrosine B free acid (Erythrosin extra bluish free acid; Tetraiodofluorescein) is a visible red dye and insecticide with colorimetric and fluorescent properties. Erythrosine B free acid serves as a membrane-impermeant live-cell dye for Gram-positive and Gram-negative bacteria, primarily staining dead cells with damaged plasma membranes. Erythrosine B free acid exhibits photosensitive larvicidal toxicity against Culex pipiens quinquefasciatus larvae. Erythrosine B free acid is used in live/dead bacterial assays in colorimetric and fluorescent measurements for low-, medium-, and high-throughput experiments, as well as in research related to mosquito-borne diseases and food coloring.
For research use only. We do not sell to patients.
- Purity : 99.07%
- CAS No.: 15905-32-5
- Formula: C20H8I4O5
- Molecular Weight:835.89
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Erythrosine B free acid shows a linear correlation between its absorbance at 530 nm and the proportion of dead cells in E. coli, K. pneumoniae, and P. mirabilis populations, enabling microplate-based quantitative colorimetric viability detection[2].
Erythrosine B (5 min) free acid is a fluorescent viability dye detectable by flow cytometry that distinguishes live from dead cells across multiple bacterial species and tracks the time-dependent increase in cell death in sodium azide-treated cultures of S. marcescens[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Erythrosine B (0.25-4 ppm; added to rearing water for larval ingestion; 24-h dark feeding period prior to light exposure; light exposure duration up to 24 h) free acid exhibits approximately 16-fold greater larvicidal efficacy against fourth-instar Culex pipiens quinquefasciatus compared with conditions without dark pre-incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:fourth-instar larvae (laboratory colony)[3]
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Dosage:0.25 ppm, 0.5 ppm, 1 ppm, 2 ppm, 4 ppm, 8 ppm, 12 ppm
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Administration:added to rearing water for larval ingestion; ~15 min feeding before light exposure; up to 24 h light exposure
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Result:Showed LC50 values of 9.05 ppm at 3 h, 7.81 ppm at 4 h, 6.61 ppm at 5 h, 5.89 ppm at 6 h, 5.44 ppm at 7 h, and 2.45 ppm at 24 h of light exposure.
Exhibited LT50 values of 7.76 h at 4 ppm, 6.73 h at 8 ppm, and 2.50 h at 12 ppm.
Demonstrated greater toxicity and faster action than fluorescein and both 1:1 and 1:3 nondispersible coprecipitated combinations.
When 8% SDS was added, the results showed LC50 values of 6.86 ppm at 0.5 h, 3.89 ppm at 1 h, 2.52 ppm at 2 h, 2.06 ppm at 3 h, 1.84 ppm at 4 h, 1.66 ppm at 5 h, 1.59 ppm at 6 h, 1.48 ppm at 7 h, and 0.66 ppm at 24 h of light exposure; LT50 values of 25.05 h at 0.5 ppm, 13.87 h at 1 ppm, 5.65 h at 2 ppm, 2.15 h at 4 ppm, 1.02 h at 5 ppm, and 0.49 h at 6 ppm; and approximately 3-fold greater toxicity than the nondispersible formulation after 24 h of light exposure.
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Animal Model:fourth-instar larvae (laboratory colony)[3]
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Dosage:0.25 ppm, 0.50 ppm, 1 ppm, 2 ppm, 4 ppm
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Administration:added to rearing water for larval ingestion; 24-h dark feeding period before light exposure; up to 24 h light exposure
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Result:Showed LC50 values of 1.69 ppm at 0.75 h, 1.19 ppm at 1 h, 0.27 ppm at 2 h, 0.20 ppm at 3 h, and 0.14 ppm at 4 h of light exposure.
Exhibited LT50 values of 2.54 h at 0.25 ppm, 1.95 h at 0.50 ppm, 1.08 h at 1 ppm, 0.76 h at 2 ppm, and 0.66 h at 4 ppm.
Increased toxic effect by about 16-fold relative to the dispersible formulation without dark preincubation.
Shortened the time required to kill 50% of test larvae.
Chemical Information
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CAS No. 15905-32-5
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Appearance Solid
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Molecular Weight 835.89
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Formula C20H8I4O5
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Color Orange to red
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SMILES
O=C1OC2(C3=CC(I)=C(C(I)=C3OC4=C(C(O)=C(C=C42)I)I)O)C5=C1C=CC=C5
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Synonyms
Erythrosin extra bluish free acid; Tetraiodofluorescein
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 5.83 mg/mL (6.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (297 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1963 mL | 5.9816 mL | 11.9633 mL | 29.9082 mL |
| 5 mM | 0.2393 mL | 1.1963 mL | 2.3927 mL | 5.9816 mL |
Erythrosine B free acid Related Classifications
HY-107864 Related Classifications
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