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Cat. No.: HY-N9924

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Glucoerucin

Glucoerucin potassium is an orally active PTP1B non-competitive allosteric inhibitor (IC₅₀ = 6.07 μM) and H₂S donor that can be isolated and extracted from Eruca sativa. Glucoerucin potassium upregulates the Nrf2 pathway and promotes irisin/UCP1-mediated adipose tissue browning through conversion to erucin or acting as a sustained-release H₂S donor, thereby regulating metabolic and cardiovascular functions. Glucoerucin potassium also exerts anticancer effects by upregulating epoxide hydrolase and through non-competitive allosteric inhibition of PTP1B. Glucoerucin potassium can be used in research on obesity, cancer, type 2 diabetes, and cardiovascular and cerebrovascular diseases.

For research use only. We do not sell to patients.

CAS No.: 21973-56-8
Formula: C₁₂H₂₃NO₉S₃
Molecular Weight:421.51

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (289 KB)

Glucoerucin Chemical Structure

CAS No.: 21973-56-8

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
K562	IC₅₀	2.5 μM	Antiproliferative activity against human erythroleukemic K562 cells assessed as inhibition of cell growth after 6 days by electronic counting with a Coulter Counter using in situ myrosinase hydrolysis of glucoerucin. Antiproliferative activity against human erythroleukemic K562 cells assessed as inhibition of cell growth after 6 days by electronic counting with a Coulter Counter using in situ myrosinase hydrolysis of glucoerucin.	jf9503523
K562	IC₅₀	3.0 μM	Antiproliferative activity against human erythroleukemic K562 cells assessed as inhibition of cell growth after 6 days by electronic counting with a Coulter Counter using pre-mix myrosinase hydrolysis of glucoerucin. Antiproliferative activity against human erythroleukemic K562 cells assessed as inhibition of cell growth after 6 days by electronic counting with a Coulter Counter using pre-mix myrosinase hydrolysis of glucoerucin.	jf9503523

In Vitro

Anti-cancerOxidative StressOthers

Anti-cancer

The myrosinase hydrolysate of Glucoerucin potassium (0.1-15 µM; 6 days) (generated in situ or pre-mix) inhibits the proliferation of human erythroleukemia K562 cells, with an in situ IC₅₀ of 2.5 µM and a pre-mix IC₅₀ of 3.0 µM^[4].
Glucoerucin potassium (0.1-500 µM; 6 days) shows almost no inhibitory activity against the proliferation of human erythroleukemia K562 cells^[4].

Oxidative Stress

Glucoerucin potassium (1-25 µM; 24 h) upregulates CYP1A1 activity and CYP1A1/CYP1B1 apoprotein levels in precision-cut rat lung slices at concentrations as low as 1 µM^[3].

Others

Glucoerucin potassium (1-25 μM; 24 h) significantly increases microsomal epoxide hydrolase (EH) activity and its protein expression in rat precision-cut liver slices, but has no significant effect on UDP-glucuronosyltransferase (UDP-GT) activity^[5].
Glucoerucin potassium (1.5 g; 37 °C) is completely hydrolyzed by myrosinase from Sinapis alba to give erucin in 82% yield^[8].
Glucoerucin potassium (1 mM; 6 h) is oxidized by a 4-fold excess of H₂O₂ in water at room temperature to glucoraphanin with a conversion rate of 97%^[8].
Glucoerucin potassium (0.1-200 µM; 30 min) inhibits recombinant human PTP1B non-competitively in a cell-free pNPP assay, with an IC₅₀ of 6.07 µM and a K_i of 6.29 µM^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

cells from rat lung sections
Concentration:

1, 2.5, 10, 25 µM
Incubation Time:

24 h
Result:

Significantly upregulated the protein expression of CYP1A1 and CYP1B1.

In Vivo

Carbohydrate MetabolismLipid MetabolismCytokines & Chemokines

Carbohydrate Metabolism

Lipid Metabolism

Cytokines & Chemokines

Glucoerucin potassium (10 mg/kg; p.o.; once daily; 10 weeks) exhibits activity in limiting body weight gain, improving lipid and blood glucose profiles, reducing visceral white adipose tissue and adipocyte size, and promoting adipose browning and thermogenesis through the Irisin/UCP1 pathway in a high-fat diet-induced obese Balb/c mouse model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Balb/c mice (high-fat diet (HFD)-induced mouse obesity model)^[6]
Dosage:

10 mg/kg
Administration:

p.o.; once daily; 10 weeks
Result:

Improved glycemic and lipid metabolism, and significantly reduced triglycerides.
Decreased the deposition of visceral white adipose tissue (vWAT) and the volume of white adipocytes.
Alleviated inflammation (reduced IL-6) and oxidative stress (restored Nrf2 levels) in vWAT.
Increased the expression of PGC-1α and FNDC5 in skeletal muscle, elevated blood irisin levels, and up-regulated UCP1 expression in vWAT, promoting browning and thermogenesis.

Related Protocols :

Chemical Information

CAS No. 21973-56-8
Molecular Weight 421.51
Formula C₁₂H₂₃NO₉S₃
SMILES

CSCCCC/C(S[C@@H]1O[C@@H]([C@H]([C@@H]([C@H]1O)O)O)CO)=N/OS(=O)(O)=O
Structure Classification
- Glucosinolates
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Piragine E, et al. Eruca sativa Mill. seed extract promotes anti-obesity and hypoglycemic effects in mice fed with a high-fat diet. Phytotherapy research : PTR. 2021 Apr;35(4):1983-1990.

[2]. Citi V, et al. Anticancer properties of erucin, an H2S‐releasing isothiocyanate, on human pancreatic adenocarcinoma cells (AsPC‐1). Phytotherapy Research. 2019 Mar;33(3):845-55. [Content Brief]

[3]. Abdull Razis AF, et al. Up-regulation of cytochrome P450 and phase II enzyme systems in rat precision-cut rat lung slices by the intact glucosinolates, glucoraphanin and glucoerucin. Lung cancer (Amsterdam, Netherlands). 2011 Mar;71(3):298-305.

[4]. Nastruzzi C, et al. In vitro cytotoxic activity of some glucosinolate-derived products generated by myrosinase hydrolysis. Journal of Agricultural and Food Chemistry. 1996 Apr 18;44(4):1014-21.

[5]. Abdull Razis AF, et al. Induction of epoxide hydrolase and glucuronosyl transferase by isothiocyanates and intact glucosinolates in precision-cut rat liver slices: importance of side-chain substituent and chirality. Archives of toxicology. 2011 Aug;85(8):919-27.

[6]. Flori L, et al. Glucoerucin, Glucosinolate From Brassicaceae Vegetables, Improves the Metabolic Profile in a Murine Model of Diet-Induced Obesity. Phytotherapy research : PTR. 2026 Jul;40(7):4556-4570.

[7]. Testai L, et al. Cardiovascular benefits of Eruca sativa mill. Defatted seed meal extract: Potential role of hydrogen sulfide. Phytotherapy research : PTR. 2022 Jun;36(6):2616-2627.

[8]. Barillari J, et al. Direct antioxidant activity of purified glucoerucin, the dietary secondary metabolite contained in rocket (Eruca sativa Mill.) seeds and sprouts. Journal of agricultural and food chemistry. 2005 Apr 06;53(7):2475-82.

[9]. Ahmed NA, et al. Watercress-derived glucosinolates as potential allosteric PTP1B inhibitors: a dual in silico and in vitro study on insulin signaling modulation. BMC chemistry. 2025 Jun 19;19(1):169.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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