HDAC6-IN-88
HDAC6-IN-88 is a selective HDAC6 inhibitor with an IC50 of 28 nM. HDAC6-IN-88 directly binds to HDAC6 and prevents the deacetylation of α-tubulin (α-tubulin) and Hsp90. HDAC6-IN-88 enhances microglial phagocytosis, promotes microtubule stabilization, and inhibits NF-κB pathway activation. DAC6-IN-88 ameliorates cognitive deficits in Aβ1-42 oligomer-induced mice, reverses HDAC6-related changes in hippocampal biomarkers, and exhibits good biosafety. HDAC6-IN-88 can be used for research related to Alzheimer's disease.
For research use only. We do not sell to patients.
- CAS No.: 2891801-74-2
- Formula: C23H25ClN2O5
- Molecular Weight:444.91
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
HDAC6 28 nM (IC50) |
In Vitro
HDAC6-IN-88 (2 h) directly binds HDAC6 in tissue lysates and protects it from pronase digestion[1].
HDAC6-IN-88 (10 h) prevents cold-induced α-tubulin deacetylation in HT22 neuronal cells[1].
HDAC6-IN-88 (1-100 μM) is well tolerated by HT22 neuronal cells even at high concentrations in the CCK8 viability assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT22 neuronal cells
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Concentration:1 and 3 μM
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Incubation Time:2 h pretreatment; Aβ1-42 oligomers 8 h
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Result:The Aβ1-42 oligomer-induced decrease in acetylated α-tubulin was prevented, increasing the acetyl-α-tubulin/α-tubulin ratio relative to Aβ1-42-treated vehicle cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 N (male, 6-8 weeks, 20-30 g)[1]
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Dosage:0.5 μg/kg and 1.5 μg/kg
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Administration:intrahippocampal injection; single co-administration with Aβ1-42 oligomers
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Result:In the NOR retention phase, Aβ1-42 oligomer-treated mice had a lower discrimination index than controls; a higher discrimination index than Aβ1-42 vehicle was produced.
In the Y-maze, a higher spontaneous alternation percentage than Aβ1-42 vehicle was produced.
Hippocampal acetyl-α-tubulin increased.
Acetyl-Hsp90 was enhanced and p-NF-κB was decreased in hippocampus.
Hippocampal RNA-seq identified 797 DEGs (313 up-regulated, 484 down-regulated); 61 overlapped axon-related genes and 49 overlapped phagocytosis-related genes, with KEGG enrichment in JAK/STAT, NF-κB, Toll-like receptor, and TNF signaling pathways.
Chemical Information
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CAS No. 2891801-74-2
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Molecular Weight 444.91
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Formula C23H25ClN2O5
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SMILES
ClC1=CC=C(C(NCCCCCCC(OC)=O)=O)C2=C1C(C3=CC=CC(OC)=C3N2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC6-IN-88 Related Classifications
HY-190530 Related Classifications
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