METTL1-IN-1
METTL1-IN-1 is an oxazolo[4,5-c]pyridine-dichlorodihydroisoquinoline hybrid derivative that inhibits METTL1-WDR4 methyltransferase (IC50 = 0.013 μM). METTL1-IN-1 downregulates the m7G methylation level of intracellular tRNA and inhibits tumor cell proliferation. METTL1-IN-1 can be used for research on METTL1-related diseases, such as cancer.
For research use only. We do not sell to patients.
- CAS No.: 3085023-09-9
- Formula: C21H21Cl2N5O3
- Molecular Weight:462.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
In Vitro
METTL1-IN-1 downregulates the m7G methylation level of intracellular tRNA, with an IC50 of 0.025 μM in the cellular m7G modification MSD assay; and an IC50 of 0.395 μM in the CTG assay for inhibition of OE21 cell proliferation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 3085023-09-9
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Molecular Weight 462.33
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Formula C21H21Cl2N5O3
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SMILES
NC1=NC2=CN=CC(N3C[C@@H](OCC3)C(N4[C@H](C5=C(Cl)C=C(Cl)C=C5CC4)C)=O)=C2O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)