Netropsin dihydrochloride
Based on 1 publication(s) in Google Scholar
Netropsin dihydrochloride is a DNA minor groove binder (MGB) and antibiotic (antibiotic). Netropsin dihydrochloride exhibits antibacterial, antiparasitic, antiviral, and antitumor activities. Netropsin dihydrochloride binds with high affinity to the minor groove of A-T-rich DNA sequences, competitively blocking the binding of specific transcription factors (such as HMGA1) to target gene promoters or enhancers, thereby inhibiting chromatin loop formation and downstream gene transcription. Netropsin dihydrochloride is a Topoisomerase inhibitor that inhibits the catalytic activity of DNA Top I and II and impedes the stabilization of their DNA-protein cross-linking complexes. Netropsin dihydrochloride can be used in research on infections, cancer, and septicemia.
For research use only. We do not sell to patients.
- Purity : 98.54%
- CAS No.: 18133-22-7
- Formula: C18H28Cl2N10O3
- Molecular Weight:503.39
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Netropsin dihydrochloride
MoreAll Antibiotic Isoforms
MoreAll DNA/RNA Synthesis Isoforms
MoreAll Parasite Isoforms
MoreAll Topoisomerase Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
Top1 |
Topoisomerase II |
In Vitro
Netropsin (2 days) dihydrochloride significantly inhibits cell migration and invasion in HCT116 colorectal cancer cells[9].
Netropsin (65-70 µM) dihydrochloride inhibits VM-26 (HY-13761)-induced topoisomerase II cleavable complexes in mouse fibrosarcoma 935.1 cell nuclei, with an IC50 of 65 µM[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (wild-type, male, 6-8 weeks of age)[2]
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Dosage:25 mg/kg
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Administration:i.p.; single dose 30 min before LPS injection; observation for 90 h
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Result:WT mice injected with LPS plus netropsin exhibited a significantly higher survival rate (36% survival) at 70-90 h following injection.
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Animal Model:C57BL/6 (wild-type, male, 6-8 weeks of age)[2]
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Dosage:25 mg/kg
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Administration:i.p.; single dose 30 min before LPS injection; observation for 90 h
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Result:1400W-treated mice receiving LPS plus netropsin did not exhibit a significant improvement in survival rates compared with those treated with LPS vehicle.
Chemical Information
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CAS No. 18133-22-7
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Appearance Solid
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Molecular Weight 503.39
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Formula C18H28Cl2N10O3
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Color Off-white to yellow
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SMILES
O=C(C1=CC(NC(CNC(N)=N)=O)=CN1C)NC2=CN(C)C(C(NCCC(N)=N)=O)=C2.[H]Cl.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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PLoS Pathog
Anti-orthopoxvirus drugs inhibit lumpy skin disease virus replication by targeting viral DNA polymerase. [Abstract]2026 Jan 26;22(1):e1013903. PMID: 41587213
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (248.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 20 mg/mL (39.73 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (285 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
[2]. Grant MA, et al. Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoter. The Biochemical journal. 2009 Feb 15;418(1):103-12. [Content Brief]
[6]. Beerman TA, et al. Netropsin and bis-netropsin analogs as inhibitors of the catalytic activity of mammalian DNA topoisomerase II and topoisomerase cleavable complexes. Biochimica et biophysica acta. 1991 Aug 27;1090(1):52-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.9865 mL | 9.9327 mL | 19.8653 mL | 49.6633 mL |
| 5 mM | 0.3973 mL | 1.9865 mL | 3.9731 mL | 9.9327 mL | |
| 10 mM | 0.1987 mL | 0.9933 mL | 1.9865 mL | 4.9663 mL | |
| 15 mM | 0.1324 mL | 0.6622 mL | 1.3244 mL | 3.3109 mL | |
| 20 mM | 0.0993 mL | 0.4966 mL | 0.9933 mL | 2.4832 mL | |
| 25 mM | 0.0795 mL | 0.3973 mL | 0.7946 mL | 1.9865 mL | |
| 30 mM | 0.0662 mL | 0.3311 mL | 0.6622 mL | 1.6554 mL | |
| DMSO | 40 mM | 0.0497 mL | 0.2483 mL | 0.4966 mL | 1.2416 mL |
| 50 mM | 0.0397 mL | 0.1987 mL | 0.3973 mL | 0.9933 mL | |
| 60 mM | 0.0331 mL | 0.1655 mL | 0.3311 mL | 0.8277 mL | |
| 80 mM | 0.0248 mL | 0.1242 mL | 0.2483 mL | 0.6208 mL | |
| 100 mM | 0.0199 mL | 0.0993 mL | 0.1987 mL | 0.4966 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Netropsin dihydrochloride Related Classifications
HY-N6800A Related Classifications
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