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Cat. No.: HY-P2185

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NFF-3

NFF-3 is a MMP-3-selective fluorescent peptide substrate that is efficiently hydrolyzed by MMP-3. NFF-3 can be used in research related to MMP-3 enzyme activity detection.

For research use only. We do not sell to patients.

CAS No.: 158584-09-9
Formula: C₇₈H₁₁₀N₂₂O₂₀
Molecular Weight:1675.84

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

NFF-3 Chemical Structure

CAS No.: 158584-09-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

NFF-3 is a MMP-3-selective fluorescent peptide substrate that is efficiently hydrolyzed by MMP-3. NFF-3 can be used in research related to MMP-3 enzyme activity detection^[1]^[2]^[3].

IC₅₀ & Target

^[1]

MMP-3

All MMP Isoforms :

In Vitro

NFF-3 is a substrate that is rapidly hydrolyzed by MMP-3 in a continuous fluorescence assay^[1].
NFF-3 is hydrolyzed by MMP-3 with k_cat/K_m = 218,000 s^-1 M^-1, whereas MMP-1 and MMP-2 show no detectable hydrolysis, and MMP-9 exhibits much lower efficiency (10,100 s^-1 M^-1)^[2].
NFF-3 confers MMP-3-triggered degradability on chitosan microspheres (CNMS) in the bacterial infection microenvironment^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 158584-09-9
Molecular Weight 1675.84
Formula C₇₈H₁₁₀N₂₂O₂₀
Sequence

{Mca}-Arg-Pro-Lys-Pro-Val-Glu-{Nva}-Trp-Arg-Lys(DNP)-NH2
Sequence Shortening

{Mca}-RPKPVE-{Nva}-WR-{K(DNP)}-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cheng L, et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta pharmaceutica Sinica. B. 2021 Oct;11(10):3220-3230.

[2]. Nagase H, et al. Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3). The Journal of biological chemistry. 1994 Aug 19;269(33):20952-7.

[3]. Cheng D, et al. High-performance lung-targeted bio-responsive platform for severe colistin-resistant bacterial pneumonia therapy. Bioactive materials. 2024 May;35:517-533.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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NFF-3

All MMP Isoforms

Biological Activity

Description

IC50 & Target

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

NFF-3 Related Classifications

HY-P2185 Related Classifications

Bioz

Keywords

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