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Cat. No.: HY-187902

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Novclobiocin 101

Novclobiocin 101 is a non-halogenated chloro Novobiocin (HY-B0425) analog and a DNA gyrase inhibitor (IC₅₀ 0.9 μM) that also inhibits DNA topoisomerase IV (IC₅₀ 6 μM). Novclobiocin 101 inhibits gyrase-catalyzed DNA supercoiling and topoisomerase IV-mediated DNA decatenation. Novclobiocin 101 exhibits antibacterial activity against Bacillus subtilis and shows cytotoxicity against small cell lung cancer cells. Novclobiocin 101 can be used for research on bacterial and fungal infections as well as cancer.

For research use only. We do not sell to patients.

CAS No.: 75057-96-4
Formula: C₃₅H₃₈N₂O₁₁
Molecular Weight:662.68

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Novclobiocin 101 Chemical Structure

CAS No.: 75057-96-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

DNA Gyrase

TOPO IV

All DNA/RNA Synthesis Isoforms :

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H187	IC50	11.61 μg/mL	Cytotoxicity against human NCI-H187 small cell lung cancer cells assessed as reduction in cell viability by resazurin microplate assay. Cytotoxicity against human NCI-H187 small cell lung cancer cells assessed as reduction in cell viability by resazurin microplate assay.	j.tet.2013.07.034

In Vitro

Bacterial InfectionFungal InfectionAnti-cancer

Bacterial Infection

Novclobiocin 101 (30 min) inhibits E. coli DNA gyrase supercoiling with an IC₅₀ of 0.9 μM and inhibits E. coli topoisomerase IV decatenation in vitro with an IC₅₀ of 6 μM^[1].
Novclobiocin 101 exhibits antibacterial activity against Bacillus subtilis ATCC 14893^[1].

Fungal Infection

Novclobiocin 101 exhibits antifungal activity against Candida albicans with an IC₅₀ of 30.47 μg/mL^[2].

Anti-cancer

Novclobiocin 101 exhibits cytotoxicity against NCI-H187 human small cell lung cancer cells with an IC₅₀ of 11.61 μg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 75057-96-4
Molecular Weight 662.68
Formula C₃₅H₃₈N₂O₁₁
SMILES

OC(C1=CC=C(O[C@H]2[C@@H]([C@@H]([C@H](C(C)(O2)C)OC)OC(C3=CC=C(C)N3)=O)O)C=C1O4)=C(NC(C5=CC(C/C=C(C)\C)=C(C=C5)O)=O)C4=O
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
- Microorganism
Escherichia coli
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Flatman RH, et al. Structure-activity relationships of aminocoumarin-type gyrase and topoisomerase IV inhibitors obtained by combinatorial biosynthesis. Antimicrob Agents Chemother. 2006 Apr;50(4):1136-42.

[2]. Dramae A, et al. Antimalarial 20-membered macrolides from Streptomyces sp. BCC33756. Tetrahedron. 2013 Sep 23;69(38):8205-8.

[3]. Janzing NBM, et al. A metabolomics perspective on clorobiocin biosynthesis: discovery of bromobiocin and novel derivatives through LC-MSE-based molecular networking. Microbiol Spectr. 2024 Jul 2;12(7):e0042324.

[4]. Eustáquio AS, et al. Clorobiocin biosynthesis in Streptomyces: identification of the halogenase and generation of structural analogs. Chemistry & biology. 2003 Mar;10(3):279-88.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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