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Cat. No.: HY-130369

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NSC10010 hydrochloride

NSC10010 hydrochloride is a Mycobacterium tuberculosis ClpC1 inhibitor and differentiation inducer (IC₅₀ = 4 µM; K_d = 2.8 µM). NSC10010 hydrochloride inhibits the ATPase and proteolytic activities of ClpC1 by binding to it, and downregulates E2F1, induces DNA damage, apoptosis, and lipid droplet accumulation, thereby inhibiting breast cancer cell proliferation and Mycobacterium tuberculosis H37Rv growth. NSC10010 hydrochloride can be used for research on breast cancer and tuberculosis.

For research use only. We do not sell to patients.

CAS No.: 6286-09-5
Formula: C₃₁H₄₂Cl₂N₄O₂
Molecular Weight:573.60

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

NSC10010 hydrochloride Chemical Structure

CAS No.: 6286-09-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]^[2]

ClpC1

4 μM (IC₅₀)

ClpC1

2.8 μM (Kd)

E2F1

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	GI50	5.4 μM	Growth inhibition against human MCF-7 breast cancer cells by SRB assay. Growth inhibition against human MCF-7 breast cancer cells by SRB assay.	15450938

In Vitro

Anti-cancerApoptosisBacterial InfectionLipid MetabolismProtein/Amino Acid MetabolismOthers

Anti-cancer

NSC10010 (compound 17) (48 h) hydrochloride inhibits MTS metabolism in MCF-7 cells with an IC₅₀ of 4 μM^[1].
NSC10010 hydrochloride has an NCI-reported SRB GI₅₀ of 5.4 μM in MCF-7 cells^[1].
NSC10010 (4 μM; 48 h) hydrochloride increases the Ki67 index in MCF-7 cells and inhibits E2F-1 protein levels in MCF-7 cells at its MTS IC₅₀ concentration^[1].
NSC10010 (4 μM; 24-48 h) hydrochloride induces DNA damage in MCF-7 cells at its MTS IC₅₀ concentration^[1].
NSC10010 (48 h) hydrochloride exhibits significant toxicity toward MCF10A non-transformed mammary epithelial cells^[1].

Anti-cancer

Apoptosis

NSC10010 (72 h) hydrochloride induces apoptosis in MCF-7 cells after 72 h^[1].

Bacterial Infection

NSC10010 (100 µM) hydrochloride binds to Mtb ClpC1 with a K_d of 2.8 µM^[2].
NSC10010 (12.5 µM; 14 days) hydrochloride inhibits the growth of Mtb H37Rv, with an MIC₉₉ of 12.5 µM^[2].

Lipid Metabolism

NSC10010 (3.5 μM; 48 h) hydrochloride induces lipid droplet accumulation in MCF-7 cells^[1].

Protein/Amino Acid Metabolism

NSC10010 (compound 1) (50-100 µM; incubation at 30 °C for 1 h) hydrochloride inhibits ClpC1-mediated FITC-casein degradation, approximately 60% at 50 µM and 100% at 100 µM^[2].

Others

NSC10010 (4 μM; 10 min) hydrochloride does not directly inhibit HDAC activity in HeLa cell nuclear extracts at its MCF-7 MTS IC₅₀ concentration^[1].
NSC10010 (500 μM; 24-48 h) hydrochloride shows poor permeability across artificial lipid membranes in the PAMPA assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MCF-7 human breast cancer cells
Concentration:

MTS IC₅₀ = 4 μM
Incubation Time:

48 h
Result:

Increased the Ki67 index in MCF-7 cells and suppresses E2F-1 protein levels in MCF-7 cells at MTS IC₅₀ concentration.

Related Protocols :

Chemical Information

CAS No. 6286-09-5
Molecular Weight 573.60
Formula C₃₁H₄₂Cl₂N₄O₂
SMILES

CC1=NC2=CC=C(OC)C=C2C(NCCCCCCCCCNC3=CC(C)=NC4=CC=C(OC)C=C34)=C1.[H]Cl.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Martirosyan AR, et al. Differentiation-inducing quinolines as experimental breast cancer agents in the MCF-7 human breast cancer cell model. Biochemical pharmacology. 2004 Nov 01;68(9):1729-38.

[2]. Bhanot A, et al. Discovery of small molecule inhibitors of Mycobacterium tuberculosis ClpC1: SAR studies and antimycobacterial evaluation. Results in Chemistry. 2023 Jan 1;5:100904.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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NSC10010 hydrochloride

All DNA/RNA Synthesis Isoforms