Perfluorobutanesulfonamide
Based on 1 Customer Validation
Perfluorobutanesulfonamide (FBSA) is a per- and polyfluoroalkyl substance (PFAS) and bovine carbonic anhydrase inhibitor with an IC50 of 1.486 µM. Perfluorobutanesulfonamide can be found as a contaminant in wastewater. Perfluorobutanesulfonamide inhibits the reversible CO2 hydration reaction catalyzed by zinc metalloenzymes. Perfluorobutanesulfonamide bioaccumulates in earthworms, is lethal to earthworms, and impairs avoidance of light. Perfluorobutanesulfonamide exhibits developmental toxicity in zebrafish embryos. Perfluorobutanesulfonamide can be used in research related to PFAS environmental chemistry and ecotoxicology.
For research use only. We do not sell to patients.
- Purity : 98.56%
- CAS No.: 30334-69-1
- Formula: C4H2F9NO2S
- Molecular Weight:299.11
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:tropical 5D wild type (specific pathogen-free colony; maintained at 28 °C, pH 7.4, 14:10 h light:dark; embryo medium; mean 48 hpf embryo wet weight 0.27 mg)[4]
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Dosage:1.0, 2.7, 6.8, 17, 37, 78, and 100 μM (developmental toxicity); 47 μM (RNA sequencing and body burden)
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Administration:static aqueous exposure in embryo medium at 8 hpf; single exposure; maintained until 24 hpf or 120 hpf for developmental toxicity and sampled at 48 hpf for RNA sequencing and body burden
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Result:At 120 hpf, this compound induced abnormal morphology, with craniofacial abnormalities and edema most frequently observed; significant % morphological effect was 50% at 37 μM, 92% at 78 μM, and 97% at 100 μM.
The lowest effect level for any morphological effect was 37 μM, and the modeled EC80 was 47 μM.
No abnormal morphology was observed at 24 hpf.
In the 120 hpf LPR assay, this compound produced hypoactivity in the dark at 17, 6.8, 2.7, and 1.0 μM; in the light, this compound produced hypoactivity at 17 and 2.7 μM and hyperactivity at 1.0 μM; LPR at 100, 78, and 37 μM was not analyzed because of high morphological effects.
No aberrant EPR behavior was observed at 24 hpf.
At 48 hpf following 47 μM this compound exposure, RNA sequencing identified 1,909 differentially expressed genes: 1,126 increased and 783 decreased.
Enriched pathways included lipid metabolism and biosynthesis, fatty acid elongation and biosynthesis, long-chain fatty-acyl-CoA biosynthesis, proteolysis, developmental processes involving brain and nervous system, ion homeostasis and transport, and immune system processes.
Forty-three transcription factors were differentially expressed; NRF2 was identified, and PPARα, RXRα, and AHR were predicted to regulate 36, 56, and 54 differentially expressed genes, respectively.
At 48 hpf following 47 μM this compound exposure, measured internal concentration was 11 ng/mg tissue and BCF was 0.80 L/kg.
Chemical Information
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CAS No. 30334-69-1
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Appearance Solid
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Molecular Weight 299.11
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Formula C4H2F9NO2S
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Color White to off-white
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SMILES
O=S(N)(C(F)(C(F)(C(F)(C(F)(F)F)F)F)F)=O
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Synonyms
FBSA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (334.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.72 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (16.72 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (291 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen YJ, et al. Emerging Perfluorobutane Sulfonamido Derivatives as a New Trend of Surfactants Used in the Semiconductor Industry. Environmental science & technology. 2024 Jan 23;58(3):1648-1658. [Content Brief]
[3]. Benfodda Z, et al. Synthesis and investigation of inhibition effect of fluorinated sulfonamide derivatives on carbonic anhydrase. European journal of medicinal chemistry. 2010 Mar;45(3):1225-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3433 mL | 16.7163 mL | 33.4325 mL | 83.5813 mL |
| 5 mM | 0.6687 mL | 3.3433 mL | 6.6865 mL | 16.7163 mL | |
| 10 mM | 0.3343 mL | 1.6716 mL | 3.3433 mL | 8.3581 mL | |
| 15 mM | 0.2229 mL | 1.1144 mL | 2.2288 mL | 5.5721 mL | |
| 20 mM | 0.1672 mL | 0.8358 mL | 1.6716 mL | 4.1791 mL | |
| 25 mM | 0.1337 mL | 0.6687 mL | 1.3373 mL | 3.3433 mL | |
| 30 mM | 0.1114 mL | 0.5572 mL | 1.1144 mL | 2.7860 mL | |
| 40 mM | 0.0836 mL | 0.4179 mL | 0.8358 mL | 2.0895 mL | |
| 50 mM | 0.0669 mL | 0.3343 mL | 0.6687 mL | 1.6716 mL | |
| 60 mM | 0.0557 mL | 0.2786 mL | 0.5572 mL | 1.3930 mL | |
| 80 mM | 0.0418 mL | 0.2090 mL | 0.4179 mL | 1.0448 mL | |
| 100 mM | 0.0334 mL | 0.1672 mL | 0.3343 mL | 0.8358 mL |
Perfluorobutanesulfonamide Related Classifications
HY-W674169 Related Classifications
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Keywords
- Perfluorobutanesulfonamide
- 30334-69-1
- FBSA
- Biochemical Assay Reagents
- Carbonic Anhydrase
- Environmental Pollutants
- CO2 hydration
- bioaccumulation
- carbonic anhydrase inhibitor
- craniofacial abnormalities
- developmental toxicity
- earthworms
- embryonic zebrafish
- larval photomotor hypoactivity
- transcriptomes
- zinc metalloenzyme
- Inhibitor
- inhibitor
- inhibit