Phos-FLAG
Phos-FLAG is a phosphine probe containing the Flag peptide. Phos-FLAG undergoes Staudinger ligation with azido groups metabolically incorporated into cell surface glycans and organ glycoproteins, enabling quantitative analysis of the presence of cell surface azides. Phos-FLAG detects azide-labeled glycans in isolated cells and organ lysates.
For research use only. We do not sell to patients.
- CAS No.: 403481-00-5
- Formula: C62H75N10O23P
- Molecular Weight:1359.29
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vivo
Phos-FLAG (16 μM; i.p.; single dose; 24 h after final Ac4ManNAz injection) successfully mediates Staudinger ligation with metabolically introduced azide groups on mouse splenocytes in vivo, generating detectable cell-surface Flag epitopes with no observed toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Es1^e/Es1^e mice (plasma esterase-deficient, male and female); B6D2F1 mice (wild-type, male and female)[1]
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Dosage:250 μM (ex vivo incubation for 1 h); 500 μM (ex vivo incubation for 6-12 h)
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Administration:ex vivo incubation; 1 h (250 μM); 6-12 h (500 μM); room temperature
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Result:Exhibited a dose-dependent increase in mean fluorescence intensity (MFI) in splenocytes from Ac4ManNAz-treated mice, with MFI values significantly higher than vehicle-treated controls.
Detected discrete labelled glycoprotein bands in heart, kidney, and liver lysates from Ac4ManNAz-treated mice, but not in brain or thymus homogenates.
Significantly reduced MFI after Phos-Flag labelling in sialidase-treated splenocytes from Ac4ManNAz-treated mice, confirming azide label presence on sialic acids.
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Animal Model:C57BL/6 mice[1]
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Dosage:16 μM
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Administration:i.p.; single dose; 24 hours after final Ac4ManNAz injection
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Result:Showed a significant increase in MFI in splenocytes from mice treated with both Ac4ManNAz and Phos-Flag relative to untreated mice or mice treated with only one agent.
Produced a larger MFI increase in ex vivo Phos-Flag treatment of splenocytes from mice given only Ac4ManNAz compared to ex vivo treatment of splenocytes from mice given both Ac4ManNAz and in vivo Phos-Flag, indicating significant in vivo Staudinger ligation of cell-surface azides.
Observed no adverse physiological effects in mice after administration.
Chemical Information
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CAS No. 403481-00-5
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Appearance Solid
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Molecular Weight 1359.29
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Formula C62H75N10O23P
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Color White to off-white
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SMILES
O=C(OC)C(C=C1)=C(P(C2=CC=CC=C2)C3=CC=CC=C3)C=C1C(N[C@@H](CC(O)=O)C(N[C@@H](CC4=CC=C(C=C4)O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 20 mg/mL (14.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7357 mL | 3.6784 mL | 7.3568 mL | 18.3920 mL |
| 5 mM | 0.1471 mL | 0.7357 mL | 1.4714 mL | 3.6784 mL | |
| 10 mM | 0.0736 mL | 0.3678 mL | 0.7357 mL | 1.8392 mL |
Phos-FLAG Related Classifications
HY-P11710 Related Classifications
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