photoCORM-1
photoCORM-1 is a visible light-induced carbon monoxide-releasing molecule and antibacterial (Antibacterial) agent. photoCORM-1 produces non-toxic byproducts and turns on fluorescence through pbt decoordination under blue, green, or red light. photoCORM-1 is internalized by cancer cells and normal cells, localizing to the cytoplasm and mitochondria. photoCORM-1 induces apoptosis (Apoptosis) under light irradiation. photoCORM-1 exhibits anticancer activity against lung cancer under light irradiation. photoCORM-1 exhibits antibacterial and antibiofilm activity against ESKAPE bacteria. photoCORM-1 can be used for research on breast cancer and LPS-induced inflammation.
For research use only. We do not sell to patients.
- CAS No.: 2892235-56-0
- Formula: C33H32Cl2N2O6
- Molecular Weight:623.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
24.22 μM
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Cytotoxicity against human A549 cells assessed as viability reduction after 419 nm illumination in the presence of 0.6 mM BSA by MTT assay.
Cytotoxicity against human A549 cells assessed as viability reduction after 419 nm illumination in the presence of 0.6 mM BSA by MTT assay.
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29983046 |
| RAW264.7 | IC50 |
40.19 μM
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Cytotoxicity against murine RAW 264.7 cells assessed as viability reduction without illumination in the presence of 0.6 mM BSA by MTT assay.
Cytotoxicity against murine RAW 264.7 cells assessed as viability reduction without illumination in the presence of 0.6 mM BSA by MTT assay.
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29983046 |
| RAW264.7 | IC50 |
12.17 μM
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Cytotoxicity against murine RAW 264.7 cells assessed as viability reduction after visible light illumination in the presence of 0.6 mM BSA by MTT assay.
Cytotoxicity against murine RAW 264.7 cells assessed as viability reduction after visible light illumination in the presence of 0.6 mM BSA by MTT assay.
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29983046 |
| L929 | IC50 |
54.34 μM
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Cytotoxicity against mouse fibroblast L929 cells assessed as viability reduction incubated 24 hrs by MTT assay.
Cytotoxicity against mouse fibroblast L929 cells assessed as viability reduction incubated 24 hrs by MTT assay.
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S0022286025000067 |
| 4T1 | IC50 |
20.43 μM
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Antiproliferative activity against mouse breast cancer 4T1 cells assessed as viability reduction incubated 24 hrs by MTT assay.
Antiproliferative activity against mouse breast cancer 4T1 cells assessed as viability reduction incubated 24 hrs by MTT assay.
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S0022286025000067 |
| MDA-MB-231 | IC50 |
21.9 μM
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Cytotoxicity against human MDA-MB-231 cells assessed as viability reduction after 15 mins of 410 nm irradiation by MTT assay.
Cytotoxicity against human MDA-MB-231 cells assessed as viability reduction after 15 mins of 410 nm irradiation by MTT assay.
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37587788 |
| MDA-MB-231 | IC50 |
202.3 μM
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Cytotoxicity against human MDA-MB-231 cells assessed as viability reduction without light irradiation by MTT assay.
Cytotoxicity against human MDA-MB-231 cells assessed as viability reduction without light irradiation by MTT assay.
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37587788 |
| A549 | IC50 |
24-36 μg/mL
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Cytotoxicity against human lung carcinoma A-549 cells assessed as viability reduction after 24 hrs incubation by MTT assay.
Cytotoxicity against human lung carcinoma A-549 cells assessed as viability reduction after 24 hrs incubation by MTT assay.
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33516984 |
In Vitro
photoCORM-1 (0-128 µg/mL; 24 h) inhibits K. pneumoniae biofilm formation at 4-8 µg/mL and inhibits A. baumannii biofilms at 32 µg/mL under blue light irradiation, whereas the biofilm MIC is 128 or >128 µg/mL in the dark[4].
photoCORM-1 releases CO under irradiation from visible to near-infrared light, with blue light being the most effective in online IR and fluorescence detection, allowing detection within 5 min at 410 nm[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-231 and HEK-293 cells
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Concentration:10, 20, 40, 50 μM
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Incubation Time:15 min at 410 nm irradiation
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Result:Without irradiation, more than 80% of MDA-MB-231 cells were viable even at 50 μM.
After photoirradiation (410 nm for 15 min), cell viability decreased to less than 20% at 50 μM.
IC50 was 21.9 μM with light irradiation (15 min, 410 nm) and 202.3 μM without light irradiation.
HEK-293 cells showed a similar viability trend.
- Research Protocol for Infectious Diseases
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- LPS-Induced Endotoxemia/Systemic Inflammation
- Annexin V plus membrane-impermeant dye apoptosis staining
- Apoptosis Solutions
- Research Protocol for Inflammation-related Diseases
- Breast Cancer Modeling
Chemical Information
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CAS No. 2892235-56-0
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Molecular Weight 623.52
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Formula C33H32Cl2N2O6
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SMILES
O=C(C1=CC2=C(N(C3=CC=C(C=C32)C(O4)=COC4=O)CC)C=C1)COC(CCCC5=CC=C(C=C5)N(CCCl)CCCl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
photoCORM-1 Related Classifications
HY-143617 Related Classifications
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